Comparison of the Migration Efficiency (Mean Value ± SD) in Response to Chemokines Between Combined Cytokine-Induced DC Precursors (Day 6)
| Chemokine (100 ng/mL) . | Stimulation . | |
|---|---|---|
| GM-CSF + SCF . | GM-CSF + SCF + TGF-β . | |
| Control | 14.15 ± 0.76 | 0 |
| MIP-1α | 25.36 ± 2.87 | 35.27 ± 0.61 |
| SLC | 78.7 ± 7.39* | 10.82 ± 5.4 |
| Chemokine (100 ng/mL) . | Stimulation . | |
|---|---|---|
| GM-CSF + SCF . | GM-CSF + SCF + TGF-β . | |
| Control | 14.15 ± 0.76 | 0 |
| MIP-1α | 25.36 ± 2.87 | 35.27 ± 0.61 |
| SLC | 78.7 ± 7.39* | 10.82 ± 5.4 |
The migration efficiency was calculated as follows. The number of the CD11c+ migrated cells was divided by that in the CD11c+ loaded cells before the migration assay.
The value of the migration efficiency in GM-CSF + SCF–stimulated HPCs is significantly larger than that in GM-SCF + SCF + TGF-β1 (P < .05).