Effect of Different Cytokines on Numbers and Viability of mBMMC and on the Expression and Release of mMCP-1
| . | Cells (×105/mL) . | % Viability . | % Mast Cells . | % mMCP-1+ . | mMCP-1 (ng/mL) . |
|---|---|---|---|---|---|
| T/I/W/S | 7.8 ± 2 | 88 ± 2.6 | 96 ± 0.8 | 90 ± 2.7 | 1,778 ± 337 |
| T/W/S | 5.3 ± 1.1 | 89 ± 2.2 | 88 ± 5.5 | 71 ± 6 | 745 ± 68 |
| T/I/W | 2.6 ± 0.4 | 75 ± 3.3 | 79 ± 2.2* | 34 ± 2.2* | 175 ± 26* |
| T/I/S | 2.7 ± 0.4 | 68 ± 3.3* | 90 ± 6† | 89 ± 1‡ | 1,866 ± 131 |
| T/W | 2.3 ± 0.4 | 72 ± 4.8 | 80 ± 2.3* | 18 ± 1.2* | 10 ± 2.4* |
| T/S | 1.2 ± 0.2* | 36 ± 4.7* | 92 ± 5.5† | 50 ± 4.1‡ | 201 ± 22* |
| I/W | 4.5 ± 0.2 | 74 ± 1.9 | 73 ± 4.5* | 1.6 ± 0.5* | 0 ± 0* |
| W/S | 10.6 ± 0.8 | 85 ± 2.8 | 94 ± 0.8 | 5 ± 1.3* | 1.3 ± 0.2* |
| . | Cells (×105/mL) . | % Viability . | % Mast Cells . | % mMCP-1+ . | mMCP-1 (ng/mL) . |
|---|---|---|---|---|---|
| T/I/W/S | 7.8 ± 2 | 88 ± 2.6 | 96 ± 0.8 | 90 ± 2.7 | 1,778 ± 337 |
| T/W/S | 5.3 ± 1.1 | 89 ± 2.2 | 88 ± 5.5 | 71 ± 6 | 745 ± 68 |
| T/I/W | 2.6 ± 0.4 | 75 ± 3.3 | 79 ± 2.2* | 34 ± 2.2* | 175 ± 26* |
| T/I/S | 2.7 ± 0.4 | 68 ± 3.3* | 90 ± 6† | 89 ± 1‡ | 1,866 ± 131 |
| T/W | 2.3 ± 0.4 | 72 ± 4.8 | 80 ± 2.3* | 18 ± 1.2* | 10 ± 2.4* |
| T/S | 1.2 ± 0.2* | 36 ± 4.7* | 92 ± 5.5† | 50 ± 4.1‡ | 201 ± 22* |
| I/W | 4.5 ± 0.2 | 74 ± 1.9 | 73 ± 4.5* | 1.6 ± 0.5* | 0 ± 0* |
| W/S | 10.6 ± 0.8 | 85 ± 2.8 | 94 ± 0.8 | 5 ± 1.3* | 1.3 ± 0.2* |
Data are expressed as the mean ± SE (n = 4 to 5, except where indicated). Bone marrow cells were grown in flasks for 1 week in WEHI (15%)/rrSCF (50 ng/mL) before they were transferred at 5 × 105/mL into 48-well plates and cultured for a further 4 days in the various cytokine combinations shown: T, rhTGF-β1 (1 ng/mL); I, rmIL-9 (5 ng/mL); W, WEHI-3B IL-3–rich supernatant (15%); S, rrSCF (50 ng/mL). The following parameters were measured 4 days after supplementation with different cytokine combinations: cell numbers and viability using nigrosin exclusion; percentage of mast cells in Leishman’s stained cytosmears; percentage of mMCP-1+ mast cells as assessed by immunohistochemical staining of cytosmears by MoAb RF 6.1; and concentrations of mMCP-1 in culture supernatants (in nanograms per milliliter) measured by ELISA using MoAb RF 6.1.
Data are significantly different (P < .03 Mann Whitney) from the control data for cells cultured in T/I/W/S.
n = 2.
n = 3.