Colony-Forming Assays With Cells From Normal or PU.1 Null Neonate Livers
| Normal | M | GM | N | Colonies/ 7,500 Cells |
| Exp. 1 | 12.3 ± 2.1 | 3.3 ± 0.6 | 6.3 ± 1.3 | 21.7 ± 3.5 |
| Exp. 2 | 21.7 ± 7.6 | 7.0 ± 1.7 | 4.7 ± 2.3 | 33.3 ± 11.0 |
| Normal | M | GM | N | Colonies/ 7,500 Cells |
| Exp. 1 | 12.3 ± 2.1 | 3.3 ± 0.6 | 6.3 ± 1.3 | 21.7 ± 3.5 |
| Exp. 2 | 21.7 ± 7.6 | 7.0 ± 1.7 | 4.7 ± 2.3 | 33.3 ± 11.0 |
| PU.1−/− | Colonies/7,500 Cells* |
| Exp. 1 | 6.4 ± 1.4 |
| Exp. 2 | 2.4 ± 0.5 |
| PU.1−/− | Colonies/7,500 Cells* |
| Exp. 1 | 6.4 ± 1.4 |
| Exp. 2 | 2.4 ± 0.5 |
Seven thousand five hundred normal and 1-1.5 × 105PU.1−/− neonate liver cells were plated in triplicate. Normal colonies were scored for macrophage (M), both macrophages and neutrophils (GM), and neutrophil (N) colonies. All values represent the means of triplicate determination +/− standard deviation.
The PU.1 null CFU plates contained only myeloid colonies.