APPENDIX
| HLA-Type . | Primers . | MgCl2 Concentration (mmol/L) . | Annealing (cycle no.) Temperature . | Probe . |
|---|---|---|---|---|
| DQB6 | DQB6B | 3.5 | 56°C (35) | DQB6E |
| (602) | (GACGTGGGGGTGTACCGC) | (TGCACACCGTGTCCAACTC) | ||
| DQB6C | ||||
| (CCGCGGAACGCCACCTC) | ||||
| DQB6 | DQB6E | 2.5 | 60°C (35) | DQB5B |
| (603) | (TGCACACCGTGTCCAACTC) | (CAGGACTTCCTTCTGGCTG) | ||
| DQB6D | ||||
| (GGAGCGCGTGCGTCTTGTA) | ||||
| DQB7 | DQB7A | 3.5 | 64°C (35) | DQB7C |
| (ATTTCGTGTACCAGTTTAAGGC) | (TGCGTTATGTGACCAGATA) | |||
| DQB7B | ||||
| (CGTGCGGAGCTCCAACTG) | ||||
| DQB8 | 2X DQB8A | 3.0 | 68°C (30) | DQB8C |
| (GTGCGTCTTGTGACCAGATA) | (AGGCGGCCCCAGCGGCGT) | |||
| 2X DQB8B | ||||
| (CTGTTCCAGTACTCGGCGG) |
| HLA-Type . | Primers . | MgCl2 Concentration (mmol/L) . | Annealing (cycle no.) Temperature . | Probe . |
|---|---|---|---|---|
| DQB6 | DQB6B | 3.5 | 56°C (35) | DQB6E |
| (602) | (GACGTGGGGGTGTACCGC) | (TGCACACCGTGTCCAACTC) | ||
| DQB6C | ||||
| (CCGCGGAACGCCACCTC) | ||||
| DQB6 | DQB6E | 2.5 | 60°C (35) | DQB5B |
| (603) | (TGCACACCGTGTCCAACTC) | (CAGGACTTCCTTCTGGCTG) | ||
| DQB6D | ||||
| (GGAGCGCGTGCGTCTTGTA) | ||||
| DQB7 | DQB7A | 3.5 | 64°C (35) | DQB7C |
| (ATTTCGTGTACCAGTTTAAGGC) | (TGCGTTATGTGACCAGATA) | |||
| DQB7B | ||||
| (CGTGCGGAGCTCCAACTG) | ||||
| DQB8 | 2X DQB8A | 3.0 | 68°C (30) | DQB8C |
| (GTGCGTCTTGTGACCAGATA) | (AGGCGGCCCCAGCGGCGT) | |||
| 2X DQB8B | ||||
| (CTGTTCCAGTACTCGGCGG) |
Sequences of Primers and Probes, as Well as Amplification Conditions for HLA Typing (Cont’d)