APPENDIX
| HLA-Type . | Primers . | MgCl2 Concentration (mmol/L) . | Annealing (cycle no.) Temperature . | Probe . |
|---|---|---|---|---|
| DR1 | DR1A | 2.5 | 64°C (30) | RIB |
| (CTTGTGGCAGCTTAAGTTTGAATG) | (GGTGCGGTTGCTGGAAAG) | |||
| DR1C | ||||
| (GGACTCCTCTTGGTTATAGATGCA) | ||||
| DR2 | DR2A | 3.5 | 70°C (35) | R2B |
| (CTGTGGCAGCCTAAGAGGGAGT) | (TTCCTGGACAGATACTTC) | |||
| DRB-B | ||||
| (CCGCTGCACTGTGAAGCTCT) | ||||
| DR3 | DR3BA | 2.5 | 56°C (38) | DRB-probe |
| (TACTTCCATAACCAGGAGGAGA) | (GCGCTTCGACAGCGACGTGGGGG) | |||
| DR3BC | ||||
| (TGCAGTAGTTGTCCACCCG) | ||||
| DR4 | DRB1-DR4A | 3.5 | 64°C (35) | DRB-3104 |
| (GTTTCTGGAGCAGGTTAAAC) | (TCTATCACCAAGAGGAGT) | |||
| DRB-B | ||||
| (CCGCTGCACTGTGAAGCTCT) | ||||
| DR7 | DRB7A | 3.5 | 64°C (35) | DRB-probe |
| (CCTGTGGCAGGGTAAGTATA) | (GCGCTTCGACAGCGACGTGGGGG) | |||
| DRB7C | ||||
| (CCCGTAGTTGTGTCTGCACAC) | ||||
| DR8 | DR8A | 3.5 | 60°C (38) | DRB-probe |
| (AGTACTCTACGGGTGAGTGTT) | (GCGCTTCGACAGCGACGTGGGGG) | |||
| DR8C | ||||
| (CTGCAGTAGGTGTCCACCAG) | ||||
| DR11 | DRB11A | 3.5 | 56°C (33) | DBB-probe |
| (GTTTCTTGGAGTACTCTACGTC) | (GCGCTTCGACAGCGACGTGGGGG) | |||
| DRB11C | ||||
| (CTGGCTGTTCCAGTACTCCT) | ||||
| DR12 | DRB8A | 2.5 | 60°C (35) | DRB-probe |
| (AGTACTCTACGGGTGAGTGTT) | (GCGCTTCGACAGCGACGTGGGGG) | |||
| DRB12C | ||||
| (CACTGTGAAGCTCTCTCCACAG) | ||||
| DR13 | DRB3A | 2.5 | 60°C (35) | DRB-probe |
| (1301, 1302) | (TACTTCCATAACCAGGAGGAGA) | (GCGCTTCGACAGCGACGTGGGGG) | ||
| DRB13C | ||||
| (CCCGCTCGTCTTCCAGGAT) | ||||
| DR15 | DR2A | 2.5 | 64°C (35) | DRB-probe |
| (CTGTGGCAGCCTAAGAGGGAGT) | (GCGCTTCGACAGCGACGTGGGGG) | |||
| DRB15C | ||||
| (CCGCGCCTGCTCCAGGAT) | ||||
| DR16 | DR2A | 2.5 | 60°C (35) | DRB-probe |
| (CTGTGGCAGCCTAAGAGGGAGT) | (GCGCTTCGACAGCGACGTGGGGG) | |||
| DRB16C | ||||
| (AGGTGTCCACCGCGGCG) | ||||
| DQB2 | DQB2A | 2.5 | 52°C-56°C (35) | DQB2B |
| (GTGCGTCTTGTGAGCAGAAG) | (CCGCCGCCCGTTTCCTCTC) | |||
| DQB2 | ||||
| (GTAGTTGTGTCTGCACACCC) | ||||
| DQB4 | DQB4A | 2.5 | 60°C (35) | DQB5D |
| (CTACTTCACCAACGGGACC) | (GAGTACGTGCGCTTCGAC) | |||
| DQB4B | ||||
| (TGGTAGTTGTGTCTGCATACG) | ||||
| DQB5 | DQB5A | 3.5 | 64°C (35) | DQB5D |
| (501) | (ACGGAGCGCGTGCGGGG) | (GAGTACGTGCGCTTCGAC) | ||
| DQB5B | ||||
| (CAGGACTTCCTTCTGGCTG) | ||||
| DQB6 | DQB6A | 3.5 | 64°C (35) | DQB6E |
| (601) | (TTTCGTGCTCCAGTTTAAGGC) | (TGCACACCGTGTCCAACTC) | ||
| DQB6C | ||||
| (CCGCGGAACGCCACCTC) |
| HLA-Type . | Primers . | MgCl2 Concentration (mmol/L) . | Annealing (cycle no.) Temperature . | Probe . |
|---|---|---|---|---|
| DR1 | DR1A | 2.5 | 64°C (30) | RIB |
| (CTTGTGGCAGCTTAAGTTTGAATG) | (GGTGCGGTTGCTGGAAAG) | |||
| DR1C | ||||
| (GGACTCCTCTTGGTTATAGATGCA) | ||||
| DR2 | DR2A | 3.5 | 70°C (35) | R2B |
| (CTGTGGCAGCCTAAGAGGGAGT) | (TTCCTGGACAGATACTTC) | |||
| DRB-B | ||||
| (CCGCTGCACTGTGAAGCTCT) | ||||
| DR3 | DR3BA | 2.5 | 56°C (38) | DRB-probe |
| (TACTTCCATAACCAGGAGGAGA) | (GCGCTTCGACAGCGACGTGGGGG) | |||
| DR3BC | ||||
| (TGCAGTAGTTGTCCACCCG) | ||||
| DR4 | DRB1-DR4A | 3.5 | 64°C (35) | DRB-3104 |
| (GTTTCTGGAGCAGGTTAAAC) | (TCTATCACCAAGAGGAGT) | |||
| DRB-B | ||||
| (CCGCTGCACTGTGAAGCTCT) | ||||
| DR7 | DRB7A | 3.5 | 64°C (35) | DRB-probe |
| (CCTGTGGCAGGGTAAGTATA) | (GCGCTTCGACAGCGACGTGGGGG) | |||
| DRB7C | ||||
| (CCCGTAGTTGTGTCTGCACAC) | ||||
| DR8 | DR8A | 3.5 | 60°C (38) | DRB-probe |
| (AGTACTCTACGGGTGAGTGTT) | (GCGCTTCGACAGCGACGTGGGGG) | |||
| DR8C | ||||
| (CTGCAGTAGGTGTCCACCAG) | ||||
| DR11 | DRB11A | 3.5 | 56°C (33) | DBB-probe |
| (GTTTCTTGGAGTACTCTACGTC) | (GCGCTTCGACAGCGACGTGGGGG) | |||
| DRB11C | ||||
| (CTGGCTGTTCCAGTACTCCT) | ||||
| DR12 | DRB8A | 2.5 | 60°C (35) | DRB-probe |
| (AGTACTCTACGGGTGAGTGTT) | (GCGCTTCGACAGCGACGTGGGGG) | |||
| DRB12C | ||||
| (CACTGTGAAGCTCTCTCCACAG) | ||||
| DR13 | DRB3A | 2.5 | 60°C (35) | DRB-probe |
| (1301, 1302) | (TACTTCCATAACCAGGAGGAGA) | (GCGCTTCGACAGCGACGTGGGGG) | ||
| DRB13C | ||||
| (CCCGCTCGTCTTCCAGGAT) | ||||
| DR15 | DR2A | 2.5 | 64°C (35) | DRB-probe |
| (CTGTGGCAGCCTAAGAGGGAGT) | (GCGCTTCGACAGCGACGTGGGGG) | |||
| DRB15C | ||||
| (CCGCGCCTGCTCCAGGAT) | ||||
| DR16 | DR2A | 2.5 | 60°C (35) | DRB-probe |
| (CTGTGGCAGCCTAAGAGGGAGT) | (GCGCTTCGACAGCGACGTGGGGG) | |||
| DRB16C | ||||
| (AGGTGTCCACCGCGGCG) | ||||
| DQB2 | DQB2A | 2.5 | 52°C-56°C (35) | DQB2B |
| (GTGCGTCTTGTGAGCAGAAG) | (CCGCCGCCCGTTTCCTCTC) | |||
| DQB2 | ||||
| (GTAGTTGTGTCTGCACACCC) | ||||
| DQB4 | DQB4A | 2.5 | 60°C (35) | DQB5D |
| (CTACTTCACCAACGGGACC) | (GAGTACGTGCGCTTCGAC) | |||
| DQB4B | ||||
| (TGGTAGTTGTGTCTGCATACG) | ||||
| DQB5 | DQB5A | 3.5 | 64°C (35) | DQB5D |
| (501) | (ACGGAGCGCGTGCGGGG) | (GAGTACGTGCGCTTCGAC) | ||
| DQB5B | ||||
| (CAGGACTTCCTTCTGGCTG) | ||||
| DQB6 | DQB6A | 3.5 | 64°C (35) | DQB6E |
| (601) | (TTTCGTGCTCCAGTTTAAGGC) | (TGCACACCGTGTCCAACTC) | ||
| DQB6C | ||||
| (CCGCGGAACGCCACCTC) |
Sequences of Primers and Probes, as Well as Amplification Conditions for HLA Typing