Sequences of the Adaptors and the Different Primers Used
| Adaptors |
| AD-L-1: 5′ CTA ATA CGA CTC ACT ATA GGG CTC GAG CGG CCG CCC GGG CAG GT 3′ |
| AD-S-1-NH2: 5′ ACC TGC CC-NH2 3′ |
| Adaptor primers |
| AP-1: 5′ CTA ATA CGA CTC ACT ATA GGG CTC GAG 3′ |
| AP-2: 5′ ATA GGG CTC GAG CGG CCG CCC 3′ |
| ALK andTPM3 gene-specific primers used on genomic DNA |
| ALK-1: 5′ GCC AGC AAA GCA GTA GTT GGG GTT G 3′ |
| ALK-2: 5′ GTC GAG GTG CGG AGC TTG CTC AGC 3′ |
| ALK-5: 5′ GAA ATG GGG AGG GAG CCA GGG AAG G 3′ |
| ALK-6: 5′ GGC TGG GTG AAC CAG CAG ACT GTG TT 3′ |
| ALK-10: 5′ GTA GAT TCT GTG TGT AAA GCC CAG CCC 3′ |
| ALK-11: 5′ TTG AAA TGT GAA ATT GCC GAG C 3′ |
| χ 1-2: 5′ GCC AAC TTC TGC CCA GGA TCT GAA TA 3′ |
| χ 1-2R: 5′ TAT TCA GAT CCT GGG CAG AAG TTG GC 3′ |
| CHRO-1-3: 5′ CCA CTA GAA AGC ATC CAA CCC GGA AC 3′ |
| CHRO-1-4: 5′ TCA ACC GTC TTC TTT CCC TCC AAT CG 3′ |
| CHRO-1-4R: 5′ CGA TTG GAG GGA AAG AAG ACG GTT GA 3′ |
| TPM 2: 5′ GCT TCT CGA GTT CAA GCG ATT CTC CTG T 3′ |
| Tropomyosin-specific primers used for RT-PCR to detect TPM3 and TPM3-ALKtranscripts |
| CTPM-1: 5′ CGA GAA GTT GAG GGA GAA AGG 3′ |
| CTPM-2: 5′ CAA CTT ACG AGC CAC CTC TTC 3′ |
| CTPM-3: 5′ CTG GCA GAG TCC CGT TGC C 3′ |
| CTPM-4: 5′ ACT GGG CGT TCT ACA TCT CAT T 3′ |
| Adaptors |
| AD-L-1: 5′ CTA ATA CGA CTC ACT ATA GGG CTC GAG CGG CCG CCC GGG CAG GT 3′ |
| AD-S-1-NH2: 5′ ACC TGC CC-NH2 3′ |
| Adaptor primers |
| AP-1: 5′ CTA ATA CGA CTC ACT ATA GGG CTC GAG 3′ |
| AP-2: 5′ ATA GGG CTC GAG CGG CCG CCC 3′ |
| ALK andTPM3 gene-specific primers used on genomic DNA |
| ALK-1: 5′ GCC AGC AAA GCA GTA GTT GGG GTT G 3′ |
| ALK-2: 5′ GTC GAG GTG CGG AGC TTG CTC AGC 3′ |
| ALK-5: 5′ GAA ATG GGG AGG GAG CCA GGG AAG G 3′ |
| ALK-6: 5′ GGC TGG GTG AAC CAG CAG ACT GTG TT 3′ |
| ALK-10: 5′ GTA GAT TCT GTG TGT AAA GCC CAG CCC 3′ |
| ALK-11: 5′ TTG AAA TGT GAA ATT GCC GAG C 3′ |
| χ 1-2: 5′ GCC AAC TTC TGC CCA GGA TCT GAA TA 3′ |
| χ 1-2R: 5′ TAT TCA GAT CCT GGG CAG AAG TTG GC 3′ |
| CHRO-1-3: 5′ CCA CTA GAA AGC ATC CAA CCC GGA AC 3′ |
| CHRO-1-4: 5′ TCA ACC GTC TTC TTT CCC TCC AAT CG 3′ |
| CHRO-1-4R: 5′ CGA TTG GAG GGA AAG AAG ACG GTT GA 3′ |
| TPM 2: 5′ GCT TCT CGA GTT CAA GCG ATT CTC CTG T 3′ |
| Tropomyosin-specific primers used for RT-PCR to detect TPM3 and TPM3-ALKtranscripts |
| CTPM-1: 5′ CGA GAA GTT GAG GGA GAA AGG 3′ |
| CTPM-2: 5′ CAA CTT ACG AGC CAC CTC TTC 3′ |
| CTPM-3: 5′ CTG GCA GAG TCC CGT TGC C 3′ |
| CTPM-4: 5′ ACT GGG CGT TCT ACA TCT CAT T 3′ |