Summary of Dissociation Constants (kd) and Numbers of Mannose Receptor Binding Sites/Cell for rGCR and pGCR
| Preparation . | Kd (nmol/L) (protein) . | Kd (nmol/L) (activity) . | Mannose R Sites/Cell (×104) (protein) . | Mannose R Sites/Cell (×104) (activity) . |
|---|---|---|---|---|
| rGCR | 17.0 | 14.5 | 5.9 | 5.0 |
| 9.4 | 10.0 | 3.2 | 3.3 | |
| 20.3 | 11.9 | 3.3 | 1.9 | |
| Mean ± SD | 15.6 ± 5.6 | 12.1 ± 2.3 | 4.1 ± 1.5 | 3.4 ± 1.6 |
| pGCR | 10.8 | 9.9 | 3.8 | 4.2 |
| 19.9 | 12.5 | 3.3 | 2.0 | |
| 14.0 | 7.6 | 2.5 | 1.4 | |
| Mean ± SD | 14.9 ± 4.6 | 10.0 ± 2.5 | 3.2 ± 0.7 | 2.5 ± 1.5 |
| rGCR and pGCR Mean ± SD | 13.2 ± 4.1 | 3.3 ± 1.3 | ||
| Preparation . | Kd (nmol/L) (protein) . | Kd (nmol/L) (activity) . | Mannose R Sites/Cell (×104) (protein) . | Mannose R Sites/Cell (×104) (activity) . |
|---|---|---|---|---|
| rGCR | 17.0 | 14.5 | 5.9 | 5.0 |
| 9.4 | 10.0 | 3.2 | 3.3 | |
| 20.3 | 11.9 | 3.3 | 1.9 | |
| Mean ± SD | 15.6 ± 5.6 | 12.1 ± 2.3 | 4.1 ± 1.5 | 3.4 ± 1.6 |
| pGCR | 10.8 | 9.9 | 3.8 | 4.2 |
| 19.9 | 12.5 | 3.3 | 2.0 | |
| 14.0 | 7.6 | 2.5 | 1.4 | |
| Mean ± SD | 14.9 ± 4.6 | 10.0 ± 2.5 | 3.2 ± 0.7 | 2.5 ± 1.5 |
| rGCR and pGCR Mean ± SD | 13.2 ± 4.1 | 3.3 ± 1.3 | ||
These calculations were made using GCR concentrations determined from micro-BCA reagent kit protein assays (protein) and from β-glucocerebrosidase enzyme activity assays (activity).