Inhibition of HUVEC Adhesion to Immobilized vWF in the Presence and Absence of Botrocetin by MoAbs Against GPIb (LJIb1 and SZ2) and Against vβ3 (LM609 and 7E3)
| MoAb . | Inhibition of HUVEC Adhesion (%) . | |
|---|---|---|
| vWF (control) . | vWF (+botrocetin) . | |
| LJIb1 | 21 ± 3.8 | 32 ± 3.2 |
| SZ2 | 16 ± 4.2 | 34 ± 4.4 |
| LM609 | 56 ± 4.3 | 55 ± 3.5 |
| 7E3 | 61 ± 2.5 | 63 ± 2.1 |
| MoAb . | Inhibition of HUVEC Adhesion (%) . | |
|---|---|---|
| vWF (control) . | vWF (+botrocetin) . | |
| LJIb1 | 21 ± 3.8 | 32 ± 3.2 |
| SZ2 | 16 ± 4.2 | 34 ± 4.4 |
| LM609 | 56 ± 4.3 | 55 ± 3.5 |
| 7E3 | 61 ± 2.5 | 63 ± 2.1 |
HUVECs were preincubated for 15 minutes on ice with antibodies against either GPIbα or with antibodies against αvβ3 at a concentration of 20 μg/mL, followed by the addition of botrocetin (10 μg/mL, final concentration). The cells were then applied to vWF-coated microplates and incubated in the presence of the antibodies for 2 hours at 37°C with 5% CO2. The results are shown as means of the percentage of inhibition of cell adhesion and standard deviations. These data represent results from three individual experiments. The difference between the inhibitory effects of LJIb1 and SZ2 on samples with and without botrocetin is at P < .05.