Table 1. PCR Primers and Conditions PCR... | American Society of Hematology

Table 1.

PCR Primers and Conditions

PCR Product	Genbank Accession	Primer Sequence (5′; 3′)	Cycle No.	Anneal Temperature (°C)
Mig	X72755 S60728	TTCCTCTTGGGCATCATCTTGCTG; GGTCTTTCAAGGATTGTAGGTGGA	35	59
IP-10	X02530 M17752	GGAACCTCCAGTCTCAGCACC; GCGTACGGTTCTAGAGAGAGGTAC	35	59
IFN-γ	V00536 AO4665	GGACCCATATGTAAAAGAAGCAGA; TGTCACTCTCCTCTTTCCAATTCT	35	57
TNF-α	M10988	TCAGCTTGAGGGTTTGCTACAA; TCTGGCCCAGGCAGTCAGATC	35	59
RANTES	M21121	GGCACGCCTCGCTGTCATCCTCA; CTTGATGTGGGCACGGGGCAGTG	35	65
MIP-1α	M25315	CTCTGCACCATGGCTCTCTGCAAC; TGTGGAATCTGCCGGGAGGTGTAG	35	62
Eotaxin	D49372	ACATGAAGGTCTCCGCAGCACTTC; TTGGCCAGGTTAAAGCAGCAGGTG	35	61
MDC	U83171	ATGGCTCGCCTACAGACTGCACTC; CACGGCAGCAGACGCTGTCTTCCA	35	62
G3PDH	M33197 M32599	GCCACATCGCTAAGACACCATGGG; CCTGGTGACCAGGCGCCCAAT	35	59

PCR Product	Genbank Accession	Primer Sequence (5′; 3′)	Cycle No.	Anneal Temperature (°C)
Mig	X72755 S60728	TTCCTCTTGGGCATCATCTTGCTG; GGTCTTTCAAGGATTGTAGGTGGA	35	59
IP-10	X02530 M17752	GGAACCTCCAGTCTCAGCACC; GCGTACGGTTCTAGAGAGAGGTAC	35	59
IFN-γ	V00536 AO4665	GGACCCATATGTAAAAGAAGCAGA; TGTCACTCTCCTCTTTCCAATTCT	35	57
TNF-α	M10988	TCAGCTTGAGGGTTTGCTACAA; TCTGGCCCAGGCAGTCAGATC	35	59
RANTES	M21121	GGCACGCCTCGCTGTCATCCTCA; CTTGATGTGGGCACGGGGCAGTG	35	65
MIP-1α	M25315	CTCTGCACCATGGCTCTCTGCAAC; TGTGGAATCTGCCGGGAGGTGTAG	35	62
Eotaxin	D49372	ACATGAAGGTCTCCGCAGCACTTC; TTGGCCAGGTTAAAGCAGCAGGTG	35	61
MDC	U83171	ATGGCTCGCCTACAGACTGCACTC; CACGGCAGCAGACGCTGTCTTCCA	35	62
G3PDH	M33197 M32599	GCCACATCGCTAAGACACCATGGG; CCTGGTGACCAGGCGCCCAAT	35	59

Abbreviations: Mig, monokine induced by interferon-γ; IP-10, interferon-γ–inducible protein-10; IFN-γ, interferon-γ; TNF-α, tumor necrosis factor-α; RANTES, regulated uponactivation, normal T expressed andsecreted; MIP1-α, macrophage inflammatory protein 1-α; MDC, macrophage-derived chemotactic factor; G3PDH, glyceraldehyde-3-phosphate dehydrogenase.

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