Effects of IAV, E coli, or both IAV and E coli on Neutrophil Fas Ligand Expression and Neutrophil Release of Fas Ligand Into Supernatant
| Assay . | Time (h) . | Control Buffer . | IAV . | E coli . | IAV + E coli . |
|---|---|---|---|---|---|
| Neutrophil FasL | 20 | 7 ± 0.2 | 12 ± 13-150 | 10 ± 2 | 10 ± 2 |
| Neutrophil FasL + blocker | 20 | 10 ± 1 | 10 ± 2 | 10 ± 2 | 8 ± 2 |
| Supernatant FasL | 3 | 0.14 ± 0.02 | 0.14 ± 0.01 | 0.16 ± 0.01 | 0.22 ± 0.053-150 |
| Supernatant FasL | 20 | 0.9 ± 0.2 | 1.36 ± 0.13-150 | 1.3 ± 0.2 | 1.3 ± 0.3 |
| Assay . | Time (h) . | Control Buffer . | IAV . | E coli . | IAV + E coli . |
|---|---|---|---|---|---|
| Neutrophil FasL | 20 | 7 ± 0.2 | 12 ± 13-150 | 10 ± 2 | 10 ± 2 |
| Neutrophil FasL + blocker | 20 | 10 ± 1 | 10 ± 2 | 10 ± 2 | 8 ± 2 |
| Supernatant FasL | 3 | 0.14 ± 0.02 | 0.14 ± 0.01 | 0.16 ± 0.01 | 0.22 ± 0.053-150 |
| Supernatant FasL | 20 | 0.9 ± 0.2 | 1.36 ± 0.13-150 | 1.3 ± 0.2 | 1.3 ± 0.3 |
Results represent mean ± SEM of four experiments. Fas ligand expression on neutrophils and supernatant Fas ligand were measured with anti-FasL (C-20) polyclonal antibodies using flow cytometry or enzyme-linked immunosorbent assay (ELISA), respectively. Flow cytometry results are mean neutrophil fluorescence values, while ELISA results are OD450 values.
Significant increase (P ≤ .05) as compared with neutrophil treated with buffer alone.