Recovery of CD34+ Cells and SRCs Is Lower in Recipients of Cultured CB Cells
Cells Transplanted in 1° Recipients . | 1° Recipients . | No. of Human Cells Transplanted per 2° Recipient3-151 . | No. of 1° Recipients With SRCs Capable of Engrafting in 2° Recipients . | % Reconstitution in 2° Recipients3-150 . | ||
---|---|---|---|---|---|---|
No. Used for 2nd BMT . | % Human Cells in The BM3-150 . | Total (×105)3-150 . | CD34+ Cells (×104)3-150 . | |||
Noncultured | 14 | 15.5 ± 8.4 (2.1-27.7) | 5.3 ± 3.1 (0.8-10.7) | 13.9 ± 9.3 (1.4-29.7) | 2 | 6.2 ± 4.5 (0.9-11.8) |
Cultured | ||||||
1 d | 6 | 6.8 ± 5.4 (2.2-16.2) | 3.5 ± 3.1 (0.9-8.4) | 6.4 ± 5.2 (1.1-13.9) | 0 | |
2 d | 3 | 4.1 ± 1.6 (3.0-5.9) | 0.9 ± 0.4 (0.5-1.3) | 3.2 ± 1.9 (1.8-5.4) | 0 | |
3 d | 7 | 4.4 ± 2.7 (1.1-8.0) | 1.0 ± 0.7 (0.3-2.4) | 2.1 ± 1.4 (0.6-4.2) | 0 |
Cells Transplanted in 1° Recipients . | 1° Recipients . | No. of Human Cells Transplanted per 2° Recipient3-151 . | No. of 1° Recipients With SRCs Capable of Engrafting in 2° Recipients . | % Reconstitution in 2° Recipients3-150 . | ||
---|---|---|---|---|---|---|
No. Used for 2nd BMT . | % Human Cells in The BM3-150 . | Total (×105)3-150 . | CD34+ Cells (×104)3-150 . | |||
Noncultured | 14 | 15.5 ± 8.4 (2.1-27.7) | 5.3 ± 3.1 (0.8-10.7) | 13.9 ± 9.3 (1.4-29.7) | 2 | 6.2 ± 4.5 (0.9-11.8) |
Cultured | ||||||
1 d | 6 | 6.8 ± 5.4 (2.2-16.2) | 3.5 ± 3.1 (0.9-8.4) | 6.4 ± 5.2 (1.1-13.9) | 0 | |
2 d | 3 | 4.1 ± 1.6 (3.0-5.9) | 0.9 ± 0.4 (0.5-1.3) | 3.2 ± 1.9 (1.8-5.4) | 0 | |
3 d | 7 | 4.4 ± 2.7 (1.1-8.0) | 1.0 ± 0.7 (0.3-2.4) | 2.1 ± 1.4 (0.6-4.2) | 0 |