Table 3.

Recovery of CD34+ Cells and SRCs Is Lower in Recipients of Cultured CB Cells

Cells Transplanted in 1° Recipients1° Recipients No. of Human Cells Transplanted per 2° Recipient3-151No. of 1° Recipients With SRCs Capable of Engrafting in 2° Recipients % Reconstitution in 2° Recipients3-150
No. Used for 2nd BMT % Human Cells in The BM3-150Total (×105)3-150CD34+ Cells (×104)3-150
Noncultured  14 15.5 ± 8.4 (2.1-27.7)  5.3 ± 3.1 (0.8-10.7) 13.9 ± 9.3 (1.4-29.7)  2  6.2 ± 4.5 (0.9-11.8) 
Cultured  
 1 d  6  6.8 ± 5.4 (2.2-16.2) 3.5 ± 3.1 (0.9-8.4)  6.4 ± 5.2 (1.1-13.9)  
 2 d  3  4.1 ± 1.6 (3.0-5.9) 0.9 ± 0.4 (0.5-1.3)  3.2 ± 1.9 (1.8-5.4)  
 3 d  7  4.4 ± 2.7 (1.1-8.0) 1.0 ± 0.7 (0.3-2.4)  2.1 ± 1.4 (0.6-4.2)  
Cells Transplanted in 1° Recipients1° Recipients No. of Human Cells Transplanted per 2° Recipient3-151No. of 1° Recipients With SRCs Capable of Engrafting in 2° Recipients % Reconstitution in 2° Recipients3-150
No. Used for 2nd BMT % Human Cells in The BM3-150Total (×105)3-150CD34+ Cells (×104)3-150
Noncultured  14 15.5 ± 8.4 (2.1-27.7)  5.3 ± 3.1 (0.8-10.7) 13.9 ± 9.3 (1.4-29.7)  2  6.2 ± 4.5 (0.9-11.8) 
Cultured  
 1 d  6  6.8 ± 5.4 (2.2-16.2) 3.5 ± 3.1 (0.9-8.4)  6.4 ± 5.2 (1.1-13.9)  
 2 d  3  4.1 ± 1.6 (3.0-5.9) 0.9 ± 0.4 (0.5-1.3)  3.2 ± 1.9 (1.8-5.4)  
 3 d  7  4.4 ± 2.7 (1.1-8.0) 1.0 ± 0.7 (0.3-2.4)  2.1 ± 1.4 (0.6-4.2)  

Presented are the 2° BMT combined from 3 experiments.

F3-150

Mean ± SD (range).

F3-151

Each 2° recipient received the equivalent of 20% of the femur of the 1° recipient.

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