Immunophenotype of Freshly Isolated B-CLL Cells
| Patient . | Recognition . | Adhesion . | Costimulation . | ||||
|---|---|---|---|---|---|---|---|
| MHC I . | MHC II . | ICAM-1 . | LFA-3 . | B7-1 . | B7-2 . | CD40 . | |
| CLL-1 | ND | +++ | − | − | − | + | + |
| CLL-2 | ND | +++ | − | − | − | + | + |
| CLL-3 | ND | +++ | − | − | − | + | + |
| CLL-4 | ND | +++ | ++ | + | − | + | ++ |
| CLL-5 | ++++ | +++ | ++ | ++ | − | ++ | ++ |
| CLL-6 | +++ | +++ | ++ | ++ | − | − | ++ |
| CLL-7 | ND | +++ | ++ | ++ | − | − | + |
| CLL-8 | +++ | +++ | ++ | ++ | + | ++ | ++ |
| CLL-9 | ++++ | +++ | ++ | ++ | + | ++ | ++ |
| CLL-10 | ++++ | +++ | +++ | ++ | + | ++ | ++ |
| CLL-11 | +++ | +++ | ++ | ++ | + | ++ | + |
| CLL-12 | ++++ | +++ | ++ | ++ | − | + | + |
| Patient . | Recognition . | Adhesion . | Costimulation . | ||||
|---|---|---|---|---|---|---|---|
| MHC I . | MHC II . | ICAM-1 . | LFA-3 . | B7-1 . | B7-2 . | CD40 . | |
| CLL-1 | ND | +++ | − | − | − | + | + |
| CLL-2 | ND | +++ | − | − | − | + | + |
| CLL-3 | ND | +++ | − | − | − | + | + |
| CLL-4 | ND | +++ | ++ | + | − | + | ++ |
| CLL-5 | ++++ | +++ | ++ | ++ | − | ++ | ++ |
| CLL-6 | +++ | +++ | ++ | ++ | − | − | ++ |
| CLL-7 | ND | +++ | ++ | ++ | − | − | + |
| CLL-8 | +++ | +++ | ++ | ++ | + | ++ | ++ |
| CLL-9 | ++++ | +++ | ++ | ++ | + | ++ | ++ |
| CLL-10 | ++++ | +++ | +++ | ++ | + | ++ | ++ |
| CLL-11 | +++ | +++ | ++ | ++ | + | ++ | + |
| CLL-12 | ++++ | +++ | ++ | ++ | − | + | + |
Freshly isolated B-CLL cells (>95%) typically coexpressed CD19 and CD5 surface markers. Mean intensity of fluorescence: −, negative; +, >0.2-0.5 logarithm; ++, 0.5-1.5 logarithm; +++, 1.5-2.5 logarithm; ++++, >2.5 logarithm; ND, not done.