Table 1. Homing of Ex Vivo–Expanded... | American Society of Hematology

Table 1.

Homing of Ex Vivo–Expanded Progenitor Cells In Vivo

Experiment No.	No. Transplanted (mean ± SEM)		No. Recovered per Organ
	No. Transplanted (mean ± SEM)		PKH26⁺ Cells				PKH26⁺CFCs (mean ± SEM)
	PKH26⁺ Cells	PKH26⁺ CFCs	Marrow	% Input	Spleen	% Input	Marrow	% Input	Spleen	% Input
1	1.2 × 10⁷	356,000 ± 92,000	91,800	0.77	37,400	0.31	190 ± 100	0.054	120 ± 90	0.032
2	8.8 × 10⁶	433,130 ± 35,000	27,500	0.31	58,800	0.67	2,870 ± 650	0.66	6,730 ± 270	1.55
3	9.7 × 10⁶	503,360 ± 43,560	50,400	0.52	169,520	1.75	4,870 ± 220	0.97	17,220 ± 140	3.42
Pooled				0.53 ± 0.13		0.91 ± 0.43		0.56 ± 0.27		1.67 ± 0.98

Experiment No.	No. Transplanted (mean ± SEM)		No. Recovered per Organ
	No. Transplanted (mean ± SEM)		PKH26⁺ Cells				PKH26⁺CFCs (mean ± SEM)
	PKH26⁺ Cells	PKH26⁺ CFCs	Marrow	% Input	Spleen	% Input	Marrow	% Input	Spleen	% Input
1	1.2 × 10⁷	356,000 ± 92,000	91,800	0.77	37,400	0.31	190 ± 100	0.054	120 ± 90	0.032
2	8.8 × 10⁶	433,130 ± 35,000	27,500	0.31	58,800	0.67	2,870 ± 650	0.66	6,730 ± 270	1.55
3	9.7 × 10⁶	503,360 ± 43,560	50,400	0.52	169,520	1.75	4,870 ± 220	0.97	17,220 ± 140	3.42
Pooled				0.53 ± 0.13		0.91 ± 0.43		0.56 ± 0.27		1.67 ± 0.98

Unfractionated (experiment 1) or Sca-1⁺c-kit⁺Lin⁻(experiments 2 and 3) BM cells were cultured for 9 days in serum-free medium supplemented with IL-3, IL-6, G-CSF, and SCF, or IL-6, IL-11, G-CSF, SCF, FLK, and TPO, respectively. Expanded cells were stained with PKH26, assayed for CFCs, and then injected IV into lethally irradiated syngeneic mice. Three hours later, marrow and spleen cells were procured and counted to determine total-organ cellularity. PKH26⁺ cells were quantitated by FACS and then sorted and assayed for CFCs. The percent recovery of input cells and CFCs in each organ was calculated as described in the legend to Fig 2.

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