Table 1. Evaluation of Gene Transfer and... | American Society of Hematology

Table 1.

Evaluation of Gene Transfer and Proviral Gene Expression Into Canine MPS I LTMC Adherent Layers and Individual CFU-GM Derived From Transduced LTMCs

Dogs	Vectors	Adherent Layer Cells			Individual LTMC-Derived CFU-GM
Dogs	Vectors	Proviral PCR	α-ID Activity	RT-PCR	Proviral PCR^*	α-ID Activity
M2	M48αID	Positive	>10 U^†	Positive	62%	17%
	LCαIDSN				(13/21)	(5/30)
M3	M48αID	Positive	101 U	Positive	27%	8%
					(4/15)	(2/25)
M4	M48αID	Positive	84 U	Positive	20%	16%
					(3/15)	(5/32)
M5	M48αID	Positive	198 U	Positive	24%	20%
					(5/21)	(2/10)
M6	LCαIDSN	Positive	100 U	Positive	31%	ND
	M48αID				(13/42)
Untransduced control		Negative	0 U	Negative	0/20	0/12

Dogs	Vectors	Adherent Layer Cells			Individual LTMC-Derived CFU-GM
Dogs	Vectors	Proviral PCR	α-ID Activity	RT-PCR	Proviral PCR^*	α-ID Activity
M2	M48αID	Positive	>10 U^†	Positive	62%	17%
	LCαIDSN				(13/21)	(5/30)
M3	M48αID	Positive	101 U	Positive	27%	8%
					(4/15)	(2/25)
M4	M48αID	Positive	84 U	Positive	20%	16%
					(3/15)	(5/32)
M5	M48αID	Positive	198 U	Positive	24%	20%
					(5/21)	(2/10)
M6	LCαIDSN	Positive	100 U	Positive	31%	ND
	M48αID				(13/42)
Untransduced control		Negative	0 U	Negative	0/20	0/12

Abbreviation: ND, sample not done.

CFU positive by PCR amplification of proviral neo^r or α-ID sequences.

Units are nanomoles per milligram per hour (normal activity ∼1 U).