Cytokine and Nitrite Production in Cocultures of HIV-1–Infected MDM and Human Fetal Astrocytes
| Days After Infection . | RT Activity (cpm) . | IFN-γ (IU/mL) . | IL-1β (pg/mL) . | TNF-α (pg/mL) . | Nitrite (μmol/L) . |
|---|---|---|---|---|---|
| 3 | 423 | <0.08 | <5.0 | <5.0 | 1.1 |
| 9 | 1,412 | <0.08 | <5.0 | <5.0 | 1.5 |
| 15 | 7,120 | <0.08 | <5.0 | <5.0 | 1.2 |
| 21 | 6,307 | <0.08 | <5.0 | <5.0 | 10.1 |
| 27 | 4,938 | <0.08 | <5.0 | <5.0 | 7.6 |
| Days After Infection . | RT Activity (cpm) . | IFN-γ (IU/mL) . | IL-1β (pg/mL) . | TNF-α (pg/mL) . | Nitrite (μmol/L) . |
|---|---|---|---|---|---|
| 3 | 423 | <0.08 | <5.0 | <5.0 | 1.1 |
| 9 | 1,412 | <0.08 | <5.0 | <5.0 | 1.5 |
| 15 | 7,120 | <0.08 | <5.0 | <5.0 | 1.2 |
| 21 | 6,307 | <0.08 | <5.0 | <5.0 | 10.1 |
| 27 | 4,938 | <0.08 | <5.0 | <5.0 | 7.6 |
Human monocytes were precultured for 8 days, infected with HIV-1ADA, and cocultured with human fetal astrocytes. Cells were fed every 3 days. Harvested supernatants were analyzed for cytokine and nitrite production as well as RT activity.