Effects of Antiasialo GM1 and Anti–NK 1.1 Antibodies on IL-12 Regulation of Angiogenesis In Vivo
| Additions to Matrigel . | Surface Area Occupied by Cells (μm2/1.26 × 105 μm2) (±SD) . | Inhibition (%) . |
|---|---|---|
| BALB/c nude | ||
| None | 484 (±179) | — |
| bFGF | 9,329 (±1,476) | — |
| bFGF + IL-12 | 2,989 (±638) | 67.9 |
| bFGF + antiasialo GM1 | 10,269 (±2,302) | — |
| bFGF + IL-12 + antiasialo GM1 | 8,638 (±1,688) | 7.4 |
| B57BL/6 nude | ||
| None | 565 (±89) | — |
| bFGF | 13,472 (±1,118) | — |
| bFGF + IL-12 | 3,112 (±389) | 76.9 |
| bFGF + anti–NK 1.1 | 12,363 (±3,347) | — |
| bFGF + IL-12 + anti–NK 1.1 | 10,361 (±604) | 2.3 |
| Additions to Matrigel . | Surface Area Occupied by Cells (μm2/1.26 × 105 μm2) (±SD) . | Inhibition (%) . |
|---|---|---|
| BALB/c nude | ||
| None | 484 (±179) | — |
| bFGF | 9,329 (±1,476) | — |
| bFGF + IL-12 | 2,989 (±638) | 67.9 |
| bFGF + antiasialo GM1 | 10,269 (±2,302) | — |
| bFGF + IL-12 + antiasialo GM1 | 8,638 (±1,688) | 7.4 |
| B57BL/6 nude | ||
| None | 565 (±89) | — |
| bFGF | 13,472 (±1,118) | — |
| bFGF + IL-12 | 3,112 (±389) | 76.9 |
| bFGF + anti–NK 1.1 | 12,363 (±3,347) | — |
| bFGF + IL-12 + anti–NK 1.1 | 10,361 (±604) | 2.3 |
Mice (BALB/c or C57BL/6 nudes) were injected subcutaneously with Matrigel alone, Matrigel + bFGF, Matrigel + bFGF + IL-12, or Matrigel + bFGF + IL-12 + rabbit antiasialo GM1 (BALB/c mice) or anti–NK 1.1 (C57BL/6 mice) antibodies. The mice injected with Matrigel containing antiasialo GM1 or anti–NK 1.1 antibodies were also treated with antiasialo GM1 (1 mg/mouse) or anti–NK 1.1 (100 mg/mouse) antibodies IP, 1 day before as well as 3 days after Matrigel injection. The results reflect the mean (±SD) surface area occupied by cells (μm2 area/1.26 × 105 μm2area) from 12 to 15 fields in each Matrigel plug, 5 Matrigel plugs/group; 4 independent experiments with antiasialo GM1 and 1 experiment with anti–NK 1.1 were performed. Determinations of surface area were performed by a semiautomated digitized analyzer.