Effect of mPEG-Derivatization on Human RBC Indices (N = 3)
| . | Control . | pH Control . | mPEG Concentration . | |||
|---|---|---|---|---|---|---|
| 1.2 mmol/L . | 2.4 mmol/L . | 5.0 mmol/L . | 10 mmol/L . | |||
| MCV (fL) | 83.7 ± 0.6 | 83.7 ± 1.5 | 83.7 ± 0.6 | 83.7 ± 0.6 | 83.7 ± 0.6 | 85.0 ± 0.0 |
| MCH (pg) | 29.7 ± 1.2 | 29.0 ± 1.0 | 30.3 ± 0.6 | 30.0 ± 0.0 | 29.0 ± 0.0 | 30.3 ± 0.6 |
| MCHC (g/dL) | 35.4 ± 14.0 | 34.7 ± 1.7 | 36.1 ± 6.1 | 35.9 ± 4.2 | 34.8 ± 4.0 | 35.7 ± 8.5 |
| RDW | 8.3 ± 0.6 | 8.1 ± 0.8 | 8.2 ± 0.1 | 8.9 ± 0.8 | 8.2 ± 0.2 | 8.5 ± 0.4 |
| Potassium (mmol K+/l RBC) | 90.5 ± 3.5 | 87.4 ± 5.0 | 83.9 ± 4.4 | 83.7 ± 4.4 | 81.8 ± 3.5 | ND |
| Sodium (mmol Na+/l RBC) | 16.0 ± 1.4 | 19.3 ± 0.1 | 19.6 ± 0.8 | 20.2 ± 2.0 | 23.7 ± 0.6* | ND |
| Magnesium (mmol Mg2+/l RBC) | 2.1 ± 0.1 | 2.0 ± 0.1 | 2.0 ± 0.0 | 2.0 ± 0.0 | 2.0 ± 0.0 | ND |
| . | Control . | pH Control . | mPEG Concentration . | |||
|---|---|---|---|---|---|---|
| 1.2 mmol/L . | 2.4 mmol/L . | 5.0 mmol/L . | 10 mmol/L . | |||
| MCV (fL) | 83.7 ± 0.6 | 83.7 ± 1.5 | 83.7 ± 0.6 | 83.7 ± 0.6 | 83.7 ± 0.6 | 85.0 ± 0.0 |
| MCH (pg) | 29.7 ± 1.2 | 29.0 ± 1.0 | 30.3 ± 0.6 | 30.0 ± 0.0 | 29.0 ± 0.0 | 30.3 ± 0.6 |
| MCHC (g/dL) | 35.4 ± 14.0 | 34.7 ± 1.7 | 36.1 ± 6.1 | 35.9 ± 4.2 | 34.8 ± 4.0 | 35.7 ± 8.5 |
| RDW | 8.3 ± 0.6 | 8.1 ± 0.8 | 8.2 ± 0.1 | 8.9 ± 0.8 | 8.2 ± 0.2 | 8.5 ± 0.4 |
| Potassium (mmol K+/l RBC) | 90.5 ± 3.5 | 87.4 ± 5.0 | 83.9 ± 4.4 | 83.7 ± 4.4 | 81.8 ± 3.5 | ND |
| Sodium (mmol Na+/l RBC) | 16.0 ± 1.4 | 19.3 ± 0.1 | 19.6 ± 0.8 | 20.2 ± 2.0 | 23.7 ± 0.6* | ND |
| Magnesium (mmol Mg2+/l RBC) | 2.1 ± 0.1 | 2.0 ± 0.1 | 2.0 ± 0.0 | 2.0 ± 0.0 | 2.0 ± 0.0 | ND |
RBC were derivatized with 0 to 10 mmol/L cyanuric chloride–activated mPEG at 4°C for 30 minutes in PBS (pH 9.2).
Abbreviation: ND, not determined.
P < .05 in comparison to saline injected animals.