Effect of Octreotide on Survival in MM Cell Lines
| . | Cell Line . | Viability (%) . | Annexin†/PI− Cells (%) . | ||
|---|---|---|---|---|---|
| 0 mol/L Octreotide . | 10−6 mol/L Octreotide . | 0 mol/L Octreotide . | 10−6 mol/L Octreotide . | ||
| IL-6–independent | LP-1 | 97 | 94 | ND | ND |
| EJM | 90 | 89 | ND | ND | |
| Karpas 707 | 94 | 94 | ND | ND | |
| HL407L | 80 | 72 | 8 | 11 | |
| U-266-1984 | 93 | 91 | ND | ND | |
| IL-6–dependent | U-1958 | 81 | 72 | 7 | 11 |
| HL407E | 80 | 65 | 8 | 14 | |
| U-266-1970 | 95 | 95 | ND | ND | |
| . | Cell Line . | Viability (%) . | Annexin†/PI− Cells (%) . | ||
|---|---|---|---|---|---|
| 0 mol/L Octreotide . | 10−6 mol/L Octreotide . | 0 mol/L Octreotide . | 10−6 mol/L Octreotide . | ||
| IL-6–independent | LP-1 | 97 | 94 | ND | ND |
| EJM | 90 | 89 | ND | ND | |
| Karpas 707 | 94 | 94 | ND | ND | |
| HL407L | 80 | 72 | 8 | 11 | |
| U-266-1984 | 93 | 91 | ND | ND | |
| IL-6–dependent | U-1958 | 81 | 72 | 7 | 11 |
| HL407E | 80 | 65 | 8 | 14 | |
| U-266-1970 | 95 | 95 | ND | ND | |
Data on viability are extracted from experiments also shown in Fig4. Values are means of triplicate determinations from a representative experiment. For the apoptosis assay, cells were cultured without or with 10−6 mol/L octreotide for 48 hours, harvested, and incubated with fluorescein-conjugated annexin V and PI and analyzed in a FACScan. Values represent the percentage of cells that were annexin V–positive/PI-negative.
Abbreviation: ND, not done.