Spleen Colony Formation by Lin−Sca-1+ BM Cells Following Prolonged Expression of c-fos
| Mice . | IFN-α/β . | Day-8 CFU-S . | Day-12 CFU-S . | Day-8/Day 12 CFU-S Ratio . |
|---|---|---|---|---|
| Control | − | 5.3 ± 1.5 | 11.0 ± 3.7 | 0.48 |
| + | 7.5 ± 2.9 | 10.8 ± 2.9 | 0.69 | |
| Mx–c-fos | − | 6.4 ± 2.5 | 10.3 ± 4.2 | 0.62 |
| + | 1.0 ± 1.4 | 13.7 ± 5.1 | 0.07 |
| Mice . | IFN-α/β . | Day-8 CFU-S . | Day-12 CFU-S . | Day-8/Day 12 CFU-S Ratio . |
|---|---|---|---|---|
| Control | − | 5.3 ± 1.5 | 11.0 ± 3.7 | 0.48 |
| + | 7.5 ± 2.9 | 10.8 ± 2.9 | 0.69 | |
| Mx–c-fos | − | 6.4 ± 2.5 | 10.3 ± 4.2 | 0.62 |
| + | 1.0 ± 1.4 | 13.7 ± 5.1 | 0.07 |
Five hundred Lin−Sca-1+ BM cells from Mx–c-fos or control littermates were cultured with SCF + IL-3 + IL-6 in the presence (200 U/mL) or absence of IFN-α/β. On day 4 of culture, those cells were collected and injected into lethally irradiated mice. Spleens from the mice were removed on day 8 or day 12 after the injection, fixed in Bouin’s solution, and the number of macroscopically visible spleen colonies was determined. Results represent mean and SD of 5 mice. The data presented are representative of two independent experiments. Purified recombinant SCF was used in this experiment.