Effects of Cycloheximide on CD437-Dependent Apoptosis and Caspase Activation
| Treatment . | No. Viable Cells ×1,000 (% viability) . | Apoptotic Index (%) . | DEVD-amc Hydrolytic Activity (Fluorescence Arbitrary Units) . |
|---|---|---|---|
| Control | 589 ± 40 | 4.7 ± 1.3 | 7.7 ± 0.3 |
| (77 ± 4) | |||
| CD437 | 493 ± 17 | 47.7 ± 1.84-150 | 33.2 ± 4.84-150 |
| (74 ± 4) | |||
| CHX-25 | 653 ± 107 | 3.9 ± 0.9 | 7.5 ± 0.9 |
| (83 ± 2) | |||
| CHX-50 | 536 ± 41 | 4.7 ± 0.9 | 8.3 ± 0.7 |
| (74 ± 3) | |||
| CD437 + CHX-25 | 565 ± 33 | 34.4 ± 1.54-150 | 30.0 ± 1.54-150 |
| (76 ± 5) | |||
| CD437 + CHX-50 | 531 ± 34 | 32.0 ± 4.64-150 | 27.0 ± 1.24-150 |
| (76 ± 5) |
| Treatment . | No. Viable Cells ×1,000 (% viability) . | Apoptotic Index (%) . | DEVD-amc Hydrolytic Activity (Fluorescence Arbitrary Units) . |
|---|---|---|---|
| Control | 589 ± 40 | 4.7 ± 1.3 | 7.7 ± 0.3 |
| (77 ± 4) | |||
| CD437 | 493 ± 17 | 47.7 ± 1.84-150 | 33.2 ± 4.84-150 |
| (74 ± 4) | |||
| CHX-25 | 653 ± 107 | 3.9 ± 0.9 | 7.5 ± 0.9 |
| (83 ± 2) | |||
| CHX-50 | 536 ± 41 | 4.7 ± 0.9 | 8.3 ± 0.7 |
| (74 ± 3) | |||
| CD437 + CHX-25 | 565 ± 33 | 34.4 ± 1.54-150 | 30.0 ± 1.54-150 |
| (76 ± 5) | |||
| CD437 + CHX-50 | 531 ± 34 | 32.0 ± 4.64-150 | 27.0 ± 1.24-150 |
| (76 ± 5) |
Cells (500,000/mL) were treated with CD437 (10−6mol/L) for 6 hours following 2 hours preincubation in the absence and presence of CHX at concentrations of 25 μmol/L (CHX-25) or 50 μmol/L (CHX-50). At the end of treatment, aliquots of the cultures were stained with erythrosin for the determination of the number of viable cells and with DAPI for the determination of the apoptotic index (% of apoptotic/total number of cells). The remaining cells were obtained, homogenized, and the extract was assayed for DEVD-amc hydrolytic activity. Each value represents the mean ± SD of three replicate cultures.
Statistically higher than control following the Student’st-test (P < .01).