Table 1. Effects of CD437 on Cell... | American Society of Hematology

Table 1.

Effects of CD437 on Cell Viability and Caspase Activation in Freshly Isolated APL Blasts

Time	Treatment	No. Viable Cells ×1,000 (% viability)	DEVD-amc Hydrolytic Activity (Fluorescence Arbitrary Units)
Patient 1
16 h	Control	550 ± 85	8.3 ± 1.4
		(97 ± 2)
	CD437	490 ± 62	11.1 ± 2.0
		(86 ± 6)
68 h	Control	502 ± 120	6.3 ± 3.9
		(72 ± 11)
	CD437	160 ± 17^*	72.0 ± 20.1^*
		(58 ± 9)
Patient 2
16 h	Control	360 ± 87	2.5 ± 1.8
		(88 ± 3)
	CD437	298 ± 26	10.7 ± 1.5^*
		(89 ± 2)
84 h	Control	324 ± 54	4.1 ± 1.0
		(88 ± 2)
	CD437	136 ± 18^*	6.6 ± 1.3
		(56 ± 2)^*

Time	Treatment	No. Viable Cells ×1,000 (% viability)	DEVD-amc Hydrolytic Activity (Fluorescence Arbitrary Units)
Patient 1
16 h	Control	550 ± 85	8.3 ± 1.4
		(97 ± 2)
	CD437	490 ± 62	11.1 ± 2.0
		(86 ± 6)
68 h	Control	502 ± 120	6.3 ± 3.9
		(72 ± 11)
	CD437	160 ± 17^*	72.0 ± 20.1^*
		(58 ± 9)
Patient 2
16 h	Control	360 ± 87	2.5 ± 1.8
		(88 ± 3)
	CD437	298 ± 26	10.7 ± 1.5^*
		(89 ± 2)
84 h	Control	324 ± 54	4.1 ± 1.0
		(88 ± 2)
	CD437	136 ± 18^*	6.6 ± 1.3
		(56 ± 2)^*

Cells (600,000/mL for patient 1 and 300,000/mL for patient 2) were treated for the indicated amount of time with CD437 (10⁻⁶mol/L). At the end of treatment, aliquots of the cultures were stained with erythrosin for the determination of the number of cells and their viability. Remaining cells were harvested, homogenized, and the extract was assayed for DEVD-amc hydrolytic activity. Each value represents the mean ± SD of three replicate cultures.

Statistically significant relative to control following the Student’st-test (P < .01).

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