G1 Exit in the Presence of Nocodazole
| . | G1 Exit . | |||
|---|---|---|---|---|
| LXSN . | AS21 . | |||
| % G1 . | % Decrease . | % G1 . | % Decrease . | |
| 24 Hours | ||||
| Untreated | 65 ± 5 | − | 61 ± 3 | − |
| Low NOC | 64 ± 8 | 1.5 | 54 ± 6 | 11.5 |
| High NOC | 76 ± 6 | −17.0* | 60 ± 7 | 1.6 |
| 48 Hours | ||||
| Untreated | 70 ± 4 | − | 56 ± 4 | − |
| Low NOC | 44 ± 7 | 37.1* | 14 ± 2 | 75.0* |
| High NOC | 65 ± 11 | 7.1 | 26 ± 3 | 53.6* |
| . | G1 Exit . | |||
|---|---|---|---|---|
| LXSN . | AS21 . | |||
| % G1 . | % Decrease . | % G1 . | % Decrease . | |
| 24 Hours | ||||
| Untreated | 65 ± 5 | − | 61 ± 3 | − |
| Low NOC | 64 ± 8 | 1.5 | 54 ± 6 | 11.5 |
| High NOC | 76 ± 6 | −17.0* | 60 ± 7 | 1.6 |
| 48 Hours | ||||
| Untreated | 70 ± 4 | − | 56 ± 4 | − |
| Low NOC | 44 ± 7 | 37.1* | 14 ± 2 | 75.0* |
| High NOC | 65 ± 11 | 7.1 | 26 ± 3 | 53.6* |
G1 exit was calculated from cell cycle analysis data by determining the percentage decrease of the G0/G1 proportion of nocodazole-treated cultures compared with untreated control cultures. This reflects the proportion of cells that could progress from G0/G1 phase and enter S and G2/M phases and was used as a measure of the G0/G1 phase arresting effects of nocodazole. Data are the mean ± SD from three experiments.
Statistically different from untreated control (LXSN), P < .05.