Cytokine Production in T Cells by Activated Endothelium and IL-15
| . | Untreated . | TNF-EC . | TNF-EC + Anti–ICAM-1 . | IL-15 . | IL-15 + TNF-EC . | IL-15 + TNF-EC + Anti–ICAM-1 . |
|---|---|---|---|---|---|---|
| IL-2 | 3.4 ± 0.8 | 3.9 ± 0.9 | 3.7 ± 0.8 | 5.0 ± 1.2 | 4.1 ± 0.8 | 3.9 ± 0.7 |
| IL-4 | 5.5 ± 1.7 | 6.7 ± 2.4 | 6.9 ± 2.7 | 7.2 ± 4.1 | 8.3 ± 4.7 | 7.4 ± 4.4 |
| TNF-α | 3.4 ± 2.7 | 4.1 ± 1.9 | 4.3 ± 2.1 | 8.6 ± 3.84-150 | 7.5 ± 2.64-150 | 7.7 ± 2.94-150 |
| IFN-γ | 6.2 ± 3.4 | 11.8 ± 3.54-150 | 8.5 ± 3.2 | 26.3 ± 8.54-151 | 37.1 ± 11.54-151 | 29.6 ± 9.54-151 |
| . | Untreated . | TNF-EC . | TNF-EC + Anti–ICAM-1 . | IL-15 . | IL-15 + TNF-EC . | IL-15 + TNF-EC + Anti–ICAM-1 . |
|---|---|---|---|---|---|---|
| IL-2 | 3.4 ± 0.8 | 3.9 ± 0.9 | 3.7 ± 0.8 | 5.0 ± 1.2 | 4.1 ± 0.8 | 3.9 ± 0.7 |
| IL-4 | 5.5 ± 1.7 | 6.7 ± 2.4 | 6.9 ± 2.7 | 7.2 ± 4.1 | 8.3 ± 4.7 | 7.4 ± 4.4 |
| TNF-α | 3.4 ± 2.7 | 4.1 ± 1.9 | 4.3 ± 2.1 | 8.6 ± 3.84-150 | 7.5 ± 2.64-150 | 7.7 ± 2.94-150 |
| IFN-γ | 6.2 ± 3.4 | 11.8 ± 3.54-150 | 8.5 ± 3.2 | 26.3 ± 8.54-151 | 37.1 ± 11.54-151 | 29.6 ± 9.54-151 |
PBLs were cultured alone (untreated), or on fixed TNF-α–activated (TNF-EC) human umbilical vein endothelial cells. The assays were performed in the absence or presence of IL-15 (25 ng/mL) and/or anti–ICAM-1, where indicated. As stated in Materials and Methods, after 48 hours of culture, CD69 and intracellular cytokine expression in CD3+ cells was measured by three-color flow cytometry. The results of four independent experiments are shown as arithmetic mean ± 1 SD of the percent of cytokine-positive cells related to the percent of production of the positive control in the CD3+ population.
P < .05 compared with untreated.
P < .01 compared with untreated (Mann-Whitney Utest).