Effect of Blocking MoAb on the Expression of CD69 by PBTLs Induced by EC and IL-15
| . | No MoAb . | Anti-CD18 . | Anti-CD49d . | Anti–ICAM-1 . | Anti–VCAM-1 . | Anti–MHC-1 . |
|---|---|---|---|---|---|---|
| Medium | 6.3 ± 2.4 | 7.8 ± 2.3 | 7.5 ± 2.4 | 6.8 ± 2.8 | 7.1 ± 2.2 | 6.2 ± 2.4 |
| EC | 21.8 ± 3.73-150 | 10.1 ± 3.2 | 17.8 ± 4.43-150 | 11.8 ± 3.1 | 20.1 ± 3.53-150 | 21.6 ± 4.53-150 |
| TNF-EC | 31.3 ± 4.73-150 | 10.7 ± 3.8 | 24.5 ± 5.63-150 | 13.8 ± 3.4 | 29.3 ± 4.33-150 | 29.9 ± 5.63-150 |
| IL-15 | 16.2 ± 4.23-150 | 17.1 ± 4.53-150 | 18.3 ± 4.73-150 | 17.3 ± 4.73-150 | 16.4 ± 4.13-150 | 16.8 ± 4.23-150 |
| EC + IL-15 | 35.4 ± 6.23-151 | 21.4 ± 4.63-150 | 30.8 ± 5.83-151 | 22.4 ± 4.93-150 | 31.7 ± 5.33-151 | 33.4 ± 5.83-151 |
| TNF-EC + IL-15 | 56.1 ± 8.23-151 | 23.4 ± 4.23-150 | 49.3 ± 7.33-151 | 25.2 ± 5.43-150 | 52.4 ± 7.13-151 | 54.5 ± 7.93-151 |
| . | No MoAb . | Anti-CD18 . | Anti-CD49d . | Anti–ICAM-1 . | Anti–VCAM-1 . | Anti–MHC-1 . |
|---|---|---|---|---|---|---|
| Medium | 6.3 ± 2.4 | 7.8 ± 2.3 | 7.5 ± 2.4 | 6.8 ± 2.8 | 7.1 ± 2.2 | 6.2 ± 2.4 |
| EC | 21.8 ± 3.73-150 | 10.1 ± 3.2 | 17.8 ± 4.43-150 | 11.8 ± 3.1 | 20.1 ± 3.53-150 | 21.6 ± 4.53-150 |
| TNF-EC | 31.3 ± 4.73-150 | 10.7 ± 3.8 | 24.5 ± 5.63-150 | 13.8 ± 3.4 | 29.3 ± 4.33-150 | 29.9 ± 5.63-150 |
| IL-15 | 16.2 ± 4.23-150 | 17.1 ± 4.53-150 | 18.3 ± 4.73-150 | 17.3 ± 4.73-150 | 16.4 ± 4.13-150 | 16.8 ± 4.23-150 |
| EC + IL-15 | 35.4 ± 6.23-151 | 21.4 ± 4.63-150 | 30.8 ± 5.83-151 | 22.4 ± 4.93-150 | 31.7 ± 5.33-151 | 33.4 ± 5.83-151 |
| TNF-EC + IL-15 | 56.1 ± 8.23-151 | 23.4 ± 4.23-150 | 49.3 ± 7.33-151 | 25.2 ± 5.43-150 | 52.4 ± 7.13-151 | 54.5 ± 7.93-151 |
PBTLs preincubated for 30 minutes at 4°C with the indicated blocking MoAb were cultured alone (medium), or on fixed resting (EC) or TNF-α–activated (TNF-EC) human umbilical vein endothelial cells. Assays were performed in the absence or presence of IL-15, where indicated. After 18 hours, CD69 expression in CD3+ cells was measured by two-color flow cytometry. The results of four independent experiments are shown as arithmetic mean ± 1 SD of the percent of CD69+ cells in the CD3+ population.
P < .01 compared with medium.
P < .01 compared with IL-15 (Mann-Whitney Utest).