Induction of CD69 Expression in Different Subpopulations of CD3+ Cells in Response to Activated ECs or ICAM-1
| Treatment . | CD3+ . | CD3+CD4+ . | CD3+ CD8+ . | CD3+ CD45RO+ . |
|---|---|---|---|---|
| TNF-EC | 25.2 ± 6.8 | 21.6 ± 5.7 | 31.1 ± 7.2 | 38.7 ± 8.5 |
| ICAM-1 | 10.6 ± 4.7 | 7.1 ± 3.9 | 16.3 ± 5.1 | 18.6 ± 5.4 |
| Treatment . | CD3+ . | CD3+CD4+ . | CD3+ CD8+ . | CD3+ CD45RO+ . |
|---|---|---|---|---|
| TNF-EC | 25.2 ± 6.8 | 21.6 ± 5.7 | 31.1 ± 7.2 | 38.7 ± 8.5 |
| ICAM-1 | 10.6 ± 4.7 | 7.1 ± 3.9 | 16.3 ± 5.1 | 18.6 ± 5.4 |
PBLs were incubated alone, on fixed TNF-α–activated EC (TNF-EC), and on ICAM-1 precoated plates. After 18 hours of incubation, the expression of CD69 in CD4+, CD8+, and CD45RO+ subsets of CD3+ cells was analyzed by three-color flow cytometry. The results of five independent experiments are shown as the arithmetic mean ± 1 SD of the percent of induction of CD69 expression with regard to the basal level in the analyzed subpopulation.