Expression of CD69, CD25, and HLA-DR by PBTLs Either Adhered or Not Adhered to Resting (EC) and TNF-–Activated (TNF-EC) Human Umbilical Vein ECs
| Cell Population . | Coculture . | CD69 . | CD25 . | HLA-DR . |
|---|---|---|---|---|
| Control | 8.5 ± 2.5 | 7.1 ± 1.3 | 9.4 ± 2.3 | |
| Nonadherent | EC | 10.0 ± 3.4 | 8.0 ± 1.1 | 7.2 ± 1.1 |
| TNF-EC | 13.8 ± 3.3 | 8.5 ± 1.7 | 6.1 ± 1.7 | |
| Adherent | EC | 21.3 ± 3.2* | 7.5 ± 1.6 | 11.8 ± 2.5 |
| TNF-EC | 36.6 ± 4.4* | 7.8 ± 1.3 | 15.5 ± 2.9† |
| Cell Population . | Coculture . | CD69 . | CD25 . | HLA-DR . |
|---|---|---|---|---|
| Control | 8.5 ± 2.5 | 7.1 ± 1.3 | 9.4 ± 2.3 | |
| Nonadherent | EC | 10.0 ± 3.4 | 8.0 ± 1.1 | 7.2 ± 1.1 |
| TNF-EC | 13.8 ± 3.3 | 8.5 ± 1.7 | 6.1 ± 1.7 | |
| Adherent | EC | 21.3 ± 3.2* | 7.5 ± 1.6 | 11.8 ± 2.5 |
| TNF-EC | 36.6 ± 4.4* | 7.8 ± 1.3 | 15.5 ± 2.9† |
PBTLs were incubated alone or on resting (EC) and TNF-α–activated (TNF-EC) endothelium monolayers. After 18 hours of culture, adherent and nonadherent cells were carefully separated as stated in Materials and Methods. Control cells correspond to unfractioned PBTLs incubated with medium alone. The expression of CD69, CD25, and HLA-DR was analyzed in CD3+ cells by two-color flow cytometry. Results correspond to the arithmetic mean ± 1 SD of the percent of positive cells of three independent experiments.
P < .01 compared with control.
P < .05 compared with control (Mann-Whitney Utest).