The Effect of Anionic Polysaccharides on Hemodynamic Behavior of SS RBC in the Ex Vivo Mesocecum Vasculature
| Experimental Group . | Treatment . | n . | ΔPRU (% increase) . | ΔΔPRU . | Tpf (sec) . |
|---|---|---|---|---|---|
| 1. | i. None | 5 | 35.7 ± 12.4 | 100.0 ± 12.4 | 68.0 ± 16.0 |
| ii. CSA | 5 | 25.5 ± 8.3* | 72.0 ± 4.2 | 54.0 ± 12.4‡ | |
| 2. | i. None | 3 | 39.8 ± 12.1 | 100.0 ± 12.1 | 85.0 ± 5.0 |
| ii. CSB | 3 | 35.9 ± 14.3 | 88.9 ± 10.3 | 80.0 ± 10.0 | |
| 3. | i. None | 4 | 25.0 ± 4.3 | 100 ± 4.3 | 52.5 ± 6.5 |
| ii. Heparin | 4 | 17.0 ± 1.7† | 72.3 ± 10.4 | 44.5 ± 4.2 | |
| 4. | i. None | 4 | 37.7 ± 10.6 | 100 ± 10.6 | 86.0 ± 24.6 |
| ii. HS | 4 | 28.5 ± 7.9† | 76.7 ± 14.0 | 68.0 ± 25.6‡ | |
| 5. | i. None | 3 | 35.4 ± 9.1 | 100.0 ± 9.1 | 95.0 ± 13.2 |
| ii. LDS | 3 | 27.0 ± 6.7† | 76.7 ± 1.8 | 61.7 ± 7.6‡ | |
| 6. | i. None | 6 | 37.7 ± 8.3 | 100.0 ± 8.3 | 97.5 ± 16.7 |
| ii. HDS | 6 | 23.0 ± 7.7* | 60.5 ± 10.0 | 65.8 ± 22.0‡ | |
| 7. | i. None | 3 | 29.7 ± 6.2 | 100 ± 6.2 | 68.3 ± 27.5 |
| ii. Fucoidan | 3 | 25.4 ± 6.5 | 86.7 ± 23 | 61.7 ± 7.6 |
| Experimental Group . | Treatment . | n . | ΔPRU (% increase) . | ΔΔPRU . | Tpf (sec) . |
|---|---|---|---|---|---|
| 1. | i. None | 5 | 35.7 ± 12.4 | 100.0 ± 12.4 | 68.0 ± 16.0 |
| ii. CSA | 5 | 25.5 ± 8.3* | 72.0 ± 4.2 | 54.0 ± 12.4‡ | |
| 2. | i. None | 3 | 39.8 ± 12.1 | 100.0 ± 12.1 | 85.0 ± 5.0 |
| ii. CSB | 3 | 35.9 ± 14.3 | 88.9 ± 10.3 | 80.0 ± 10.0 | |
| 3. | i. None | 4 | 25.0 ± 4.3 | 100 ± 4.3 | 52.5 ± 6.5 |
| ii. Heparin | 4 | 17.0 ± 1.7† | 72.3 ± 10.4 | 44.5 ± 4.2 | |
| 4. | i. None | 4 | 37.7 ± 10.6 | 100 ± 10.6 | 86.0 ± 24.6 |
| ii. HS | 4 | 28.5 ± 7.9† | 76.7 ± 14.0 | 68.0 ± 25.6‡ | |
| 5. | i. None | 3 | 35.4 ± 9.1 | 100.0 ± 9.1 | 95.0 ± 13.2 |
| ii. LDS | 3 | 27.0 ± 6.7† | 76.7 ± 1.8 | 61.7 ± 7.6‡ | |
| 6. | i. None | 6 | 37.7 ± 8.3 | 100.0 ± 8.3 | 97.5 ± 16.7 |
| ii. HDS | 6 | 23.0 ± 7.7* | 60.5 ± 10.0 | 65.8 ± 22.0‡ | |
| 7. | i. None | 3 | 29.7 ± 6.2 | 100 ± 6.2 | 68.3 ± 27.5 |
| ii. Fucoidan | 3 | 25.4 ± 6.5 | 86.7 ± 23 | 61.7 ± 7.6 |
The values are mean ± SD. ΔPRU represents percent increase in PRU following infusion of RBC compared with preinfusion PRU; ΔΔPRU represents percent changes after control values are normalized to 100%; Tpf, pressure-flow recovery time to the baseline level. In these experiments, SS RBC were either untreated or incubated with a given anionic polysaccharide (250 μg/ml).
Abbreviations: CSA and CSB, chondroitin sulfates A and B; LDS, low molecular weight dextran sulfate; HDS, high molecular weight dextran sulfate.
P < .01-.001 and
P < .05 (paired t-test) compared with control (None) ΔPRU.
P < .02 (paired t-test) compared with control Tpf.