Expression of Muc-1 on Cancer Cell Lines
| Cell Line . | Type . | VU-3C6 . | DF3 . | VU-4H5 . | DF3P . |
|---|---|---|---|---|---|
| ALL207 | ALL, B-cell | 0 | 0 | 0 | 0 |
| Jurkat | ALL, T-cell | + | 0 | 0 | 0/+ |
| B15 | ALL, B-cell | 0 | 0 | 0 | 0 |
| NALM 6 | ALL, B-cell | 0 | 0 | 0 | 0 |
| HL60 | AML, Promyeloc. | 0 | 0 | 0 | 0 |
| K562 | AML, Erythroleuk. | 0 | 0 | 0 | 0 |
| HZ | B-cell, NHL | + | + | + | 0 |
| DHL6 | B-cell, NHL | 0 | 0 | 0 | 0 |
| DHL16 | B-cell, NHL | 0 | 0 | 0 | + |
| RL | B-cell, NHL | 0 | 0 | 0 | 0 |
| BCBL-1 | B-cell, PEL | 0 | ND | 0 | ND |
| MCF.7 | Breast | ++ | ++ | 0 | 0 |
| ZR-75 | Breast | ++ | ++ | 0 | 0 |
| CESS | B-lymphoblast | + | 0 | + | 0 |
| L5174T | Colon | 0 | 0 | 0 | 0 |
| SW480 | Colon | 0 | + | 0 | 0 |
| HUTU80 | Duodenal | 0 | 0 | 0 | 0 |
| T.Tu | Esophageal | 0 | ++ | 0 | 0 |
| AGSB | Gastric | 0 | 0 | 0 | 0 |
| CMK | Megakaryocytic | + | 0 | 0 | 0 |
| HS294T | Melanoma | 0 | 0 | 0 | 0 |
| AC5 | Melanoma | 0 | 0 | 0 | 0 |
| ARH-77 | Myeloma | ++ | + | + | 0 |
| HS Sultan | Myeloma | ++ | 0 | + | 0 |
| IM9 | Myeloma | ++ | + | ++ | 0 |
| OCI My-5 | Myeloma | +++ | +++ | + | + |
| S6B45 | Myeloma | +++ | +++ | ++ | + |
| RPMI 8226 | Myeloma | +++ | +++ | ++ | ++ |
| U266 | Myeloma | + | +++ | + | + |
| 36M | Ovarian | 0 | ++ | 0 | 0 |
| CAOV-3 | Ovarian | 0 | + | 0 | 0 |
| OVC3 | Ovarian | 0 | + | 0 | 0 |
| Capan-2 | Pancreas | +++ | +++ | + | ND |
| DU145 | Prostate | ++ | +++ | 0 | 0 |
| PC3 | Prostate | + | 0 | 0 | 0 |
| A498 | Renal | 0 | + | 0 | ND |
| ACHN | Renal | + | + | 0 | ND |
| Caki-1 | Renal | 0 | ++ | 0 | ND |
| 786-0 | Renal | 0 | 0 | 0 | ND |
| Cell Line . | Type . | VU-3C6 . | DF3 . | VU-4H5 . | DF3P . |
|---|---|---|---|---|---|
| ALL207 | ALL, B-cell | 0 | 0 | 0 | 0 |
| Jurkat | ALL, T-cell | + | 0 | 0 | 0/+ |
| B15 | ALL, B-cell | 0 | 0 | 0 | 0 |
| NALM 6 | ALL, B-cell | 0 | 0 | 0 | 0 |
| HL60 | AML, Promyeloc. | 0 | 0 | 0 | 0 |
| K562 | AML, Erythroleuk. | 0 | 0 | 0 | 0 |
| HZ | B-cell, NHL | + | + | + | 0 |
| DHL6 | B-cell, NHL | 0 | 0 | 0 | 0 |
| DHL16 | B-cell, NHL | 0 | 0 | 0 | + |
| RL | B-cell, NHL | 0 | 0 | 0 | 0 |
| BCBL-1 | B-cell, PEL | 0 | ND | 0 | ND |
| MCF.7 | Breast | ++ | ++ | 0 | 0 |
| ZR-75 | Breast | ++ | ++ | 0 | 0 |
| CESS | B-lymphoblast | + | 0 | + | 0 |
| L5174T | Colon | 0 | 0 | 0 | 0 |
| SW480 | Colon | 0 | + | 0 | 0 |
| HUTU80 | Duodenal | 0 | 0 | 0 | 0 |
| T.Tu | Esophageal | 0 | ++ | 0 | 0 |
| AGSB | Gastric | 0 | 0 | 0 | 0 |
| CMK | Megakaryocytic | + | 0 | 0 | 0 |
| HS294T | Melanoma | 0 | 0 | 0 | 0 |
| AC5 | Melanoma | 0 | 0 | 0 | 0 |
| ARH-77 | Myeloma | ++ | + | + | 0 |
| HS Sultan | Myeloma | ++ | 0 | + | 0 |
| IM9 | Myeloma | ++ | + | ++ | 0 |
| OCI My-5 | Myeloma | +++ | +++ | + | + |
| S6B45 | Myeloma | +++ | +++ | ++ | + |
| RPMI 8226 | Myeloma | +++ | +++ | ++ | ++ |
| U266 | Myeloma | + | +++ | + | + |
| 36M | Ovarian | 0 | ++ | 0 | 0 |
| CAOV-3 | Ovarian | 0 | + | 0 | 0 |
| OVC3 | Ovarian | 0 | + | 0 | 0 |
| Capan-2 | Pancreas | +++ | +++ | + | ND |
| DU145 | Prostate | ++ | +++ | 0 | 0 |
| PC3 | Prostate | + | 0 | 0 | 0 |
| A498 | Renal | 0 | + | 0 | ND |
| ACHN | Renal | + | + | 0 | ND |
| Caki-1 | Renal | 0 | ++ | 0 | ND |
| 786-0 | Renal | 0 | 0 | 0 | ND |
Cell surface staining of neoplastic cell lines was assessed by flow cytometry using the VU-3C6 and DF3 antibodies that identify both Muc-1 glycosylated and core protein determinants and the VU-4H5 and DF3-P antibodies that specifically identify Muc-1 core protein. Intensity of staining is denoted as follows: (0) absent; (+) low; (++) medium; (+++) high.
Abbreviation: ND, not determined.