Detection of ER4G, Rat Complement Components, and Human Ig on Kidney Biopsy Samples by Immunofluorescence Staining
| . | ER4G . | C3 . | C5b-9 . | hIgG . | hIgM . |
|---|---|---|---|---|---|
| PBS3-150 | ++3-151 | ++ | + | − | − |
| IVIgG | ++ | ± | ± | + | − |
| IVIgM | ++ | − | − | +‖±‖− | +‖±‖− |
| . | ER4G . | C3 . | C5b-9 . | hIgG . | hIgM . |
|---|---|---|---|---|---|
| PBS3-150 | ++3-151 | ++ | + | − | − |
| IVIgG | ++ | ± | ± | + | − |
| IVIgM | ++ | − | − | +‖±‖− | +‖±‖− |
Treatment 30 minutes before injection of ER4G.
Intensity of immunofluorescence staining scored as −, no stain; ±, <¼; +, ¼ to ½; and ++, >½ of glomerulus stained on the average; 20 glomeruli were scored per biopsy. Results are given per group of four rats and indicated separately for 10-minute, 30-minutes, 24-hour biopsies where time-dependent differences were observed; interindividual differences were always less than one scoring unit. Groups of four rats each were injected with either PBS, IVIgG, or IVIgM followed by anti-Thy 1 MoAb, ER4G. Biopsy samples of kidneys were taken 10 minutes, 30 minutes, and 24 hours thereafter and stained for the presence of ER4G, rat complement, and human Igs as described in Materials and Methods.