Transient Expression Assays of pEDFVIIwt, pEDFVIICys97, and pEDFVIIArg100 in COS-1 Cells
| . | FVIIwt . | FVIICys97 . | FVIIArg100 . |
|---|---|---|---|
| Cell lysate, VII:Ag | 100 ± 2.8 | 38.1 ± 3.9 | 54.6 ± 3 |
| Conditioned media, VII:Ag | 100 ± 3 | 6.6 ± 0.5 | 16.7 ± 2.1 |
| Conditioned media, VII:C | 100 ± 2.9 | 6.1 ± 0.3 | 2.8 ± 0.2 |
| . | FVIIwt . | FVIICys97 . | FVIIArg100 . |
|---|---|---|---|
| Cell lysate, VII:Ag | 100 ± 2.8 | 38.1 ± 3.9 | 54.6 ± 3 |
| Conditioned media, VII:Ag | 100 ± 3 | 6.6 ± 0.5 | 16.7 ± 2.1 |
| Conditioned media, VII:C | 100 ± 2.9 | 6.1 ± 0.3 | 2.8 ± 0.2 |
FVII levels were measured in cell lysates and conditioned media 36 hours after 8 independent transfections of cells from a single clone transfected with each construct. Results are expressed as the percentage of FVII (mean ± SE) produced by the wild-type construct.