IL-7 Binding to Cells Lacking Cell-Surface HS
| . | Mean Fluorescence Intensity of IL-7 Binding* . | |
|---|---|---|
| Control . | Heparitinase-Treated (% of control) . | |
| Hematopoietic cells | ||
| LTBMC lymphocytes | 159.70 | 31.24 (19.56) |
| F10 | 238.65 | 24.26 (10.16) |
| BC7.12 | 71.36 | 15.43 (21.62) |
| DW34 | 103.41 | 15.21 (14.71) |
| FDC-P1 | 10.15 | ND |
| Stromal cells | ||
| OP42 | 45.78 | 10.72 (23.42) |
| BMS2 | 54.06 | 13.16 (24.34) |
| BMS2.4 | 34.83 | ND |
| TEC | 36.02 | ND |
| NIH-3T3 | 66.26 | ND |
| . | Mean Fluorescence Intensity of IL-7 Binding* . | |
|---|---|---|
| Control . | Heparitinase-Treated (% of control) . | |
| Hematopoietic cells | ||
| LTBMC lymphocytes | 159.70 | 31.24 (19.56) |
| F10 | 238.65 | 24.26 (10.16) |
| BC7.12 | 71.36 | 15.43 (21.62) |
| DW34 | 103.41 | 15.21 (14.71) |
| FDC-P1 | 10.15 | ND |
| Stromal cells | ||
| OP42 | 45.78 | 10.72 (23.42) |
| BMS2 | 54.06 | 13.16 (24.34) |
| BMS2.4 | 34.83 | ND |
| TEC | 36.02 | ND |
| NIH-3T3 | 66.26 | ND |
Abbreviation: ND, not determined.
Undigested or heparitinase-digested cells were stained with biotinylated IL-7 as described in Experimental Procedures. Data are representative of 2 to 10 independent experiments, and the mean fluorescence intensity of the negative control reagent was <10 U for each cell type.