Mutation Analysis of the Lymph Node Monocytoid B Cells
| Case/ Cells . | Germline VH (reference) . | N . | % Identity . | R/S . | Exp R CDR . | Exp R FR . | PCDR . | PFR . | Germline DH . | Germline JH . | Frame . | |
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| CDR . | FR . | |||||||||||
| Case 1 | ||||||||||||
| 6-VH3 | DP77 (33) | 21 | 90.54 | 12/2 | 4/3 | — | — | — | — | D21-9 | JH6 | Out |
| 8-VH3 | DP54 (31) | 0 | 100 | 0/0 | 0/0 | — | — | — | — | DHQ52/DA1 | JH4 | In |
| 10-VH3 | DP47 (31) | 2 | 99.1 | 1/0 | 1/0 | — | — | — | — | DXP1/D21 | JH4 | In |
| 11-VH3 | COS 15 (33) | 1 | 99.5 | 0/0 | 0/1 | — | — | — | — | DM1 | JH6 | In |
| 21-VH3 | DP49 (31) | 1 | 99.5 | 0/0 | 0/1 | — | — | — | — | D2 | JH4 | Out |
| Case 2 | ||||||||||||
| 2-VH3 | DP54 (31) | 4 | 98.2 | 2/1 | 0/1 | — | — | — | — | DXP1 | JH4 | Out |
| 5-VH4 | 3d279 (32) | 10 | 95.5 | 6/1 | 0/3 | 2.5 | 5.1 | .017 | .0007 | D2 | JH4 | In |
| 11-VH4 | DP71 (31) | 1 | 99.5 | 0/0 | 0/1 | — | — | — | — | D4 | JH4 | In |
| 12-VH3 | DP47 (31) | 1 | 99.5 | 0/1 | 0/0 | — | — | — | — | DK4 | JH5 | In |
| 13-VH3 | DP54 (31) | 13 | 94.2 | 4/0 | 4/5 | 3.3 | 7 | .215 | .056 | DN4 + DXP′ 1 | JH6 | In |
| Case 3 | ||||||||||||
| 10-VH4 | DP70 (31) | 2 | 99.1 | 0/1 | 1/0 | — | — | — | — | DN1/D23-7 | JH4 | In |
| 14-VH4 | DP63/V4-34 (31) | 8 | 96.3 | 4/0 | 3/1 | — | — | — | — | DXP1 | JH4 | In (SC) |
| Case 4 | ||||||||||||
| 1-VH3 | DP35 (31) | 6 | 97.3 | 2/0 | 4/0 | 1.4 | 3.2 | .287 | .267 | NI | JH6 | In |
| 7-VH3 | DP49 (31) | 10 | 95.5 | 4/1 | 3/2 | — | — | — | — | D3 | JH4 | Out |
| 17-VH3 | DP58 (31) | 4 | 98.2 | 2/0 | 1/1 | — | — | — | — | DM1 | JH5 | Out |
| 20-VH4 | DP65/3d2773 (31) | 10 | 95.5 | 2/1 | 4/3 | 2.5 | 5.2 | .28 | .187 | DK4 | JH4 | In |
| Case/ Cells . | Germline VH (reference) . | N . | % Identity . | R/S . | Exp R CDR . | Exp R FR . | PCDR . | PFR . | Germline DH . | Germline JH . | Frame . | |
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| CDR . | FR . | |||||||||||
| Case 1 | ||||||||||||
| 6-VH3 | DP77 (33) | 21 | 90.54 | 12/2 | 4/3 | — | — | — | — | D21-9 | JH6 | Out |
| 8-VH3 | DP54 (31) | 0 | 100 | 0/0 | 0/0 | — | — | — | — | DHQ52/DA1 | JH4 | In |
| 10-VH3 | DP47 (31) | 2 | 99.1 | 1/0 | 1/0 | — | — | — | — | DXP1/D21 | JH4 | In |
| 11-VH3 | COS 15 (33) | 1 | 99.5 | 0/0 | 0/1 | — | — | — | — | DM1 | JH6 | In |
| 21-VH3 | DP49 (31) | 1 | 99.5 | 0/0 | 0/1 | — | — | — | — | D2 | JH4 | Out |
| Case 2 | ||||||||||||
| 2-VH3 | DP54 (31) | 4 | 98.2 | 2/1 | 0/1 | — | — | — | — | DXP1 | JH4 | Out |
| 5-VH4 | 3d279 (32) | 10 | 95.5 | 6/1 | 0/3 | 2.5 | 5.1 | .017 | .0007 | D2 | JH4 | In |
| 11-VH4 | DP71 (31) | 1 | 99.5 | 0/0 | 0/1 | — | — | — | — | D4 | JH4 | In |
| 12-VH3 | DP47 (31) | 1 | 99.5 | 0/1 | 0/0 | — | — | — | — | DK4 | JH5 | In |
| 13-VH3 | DP54 (31) | 13 | 94.2 | 4/0 | 4/5 | 3.3 | 7 | .215 | .056 | DN4 + DXP′ 1 | JH6 | In |
| Case 3 | ||||||||||||
| 10-VH4 | DP70 (31) | 2 | 99.1 | 0/1 | 1/0 | — | — | — | — | DN1/D23-7 | JH4 | In |
| 14-VH4 | DP63/V4-34 (31) | 8 | 96.3 | 4/0 | 3/1 | — | — | — | — | DXP1 | JH4 | In (SC) |
| Case 4 | ||||||||||||
| 1-VH3 | DP35 (31) | 6 | 97.3 | 2/0 | 4/0 | 1.4 | 3.2 | .287 | .267 | NI | JH6 | In |
| 7-VH3 | DP49 (31) | 10 | 95.5 | 4/1 | 3/2 | — | — | — | — | D3 | JH4 | Out |
| 17-VH3 | DP58 (31) | 4 | 98.2 | 2/0 | 1/1 | — | — | — | — | DM1 | JH5 | Out |
| 20-VH4 | DP65/3d2773 (31) | 10 | 95.5 | 2/1 | 4/3 | 2.5 | 5.2 | .28 | .187 | DK4 | JH4 | In |
Abbreviations: VH gene, IgH variable gene; DH gene, IgH diversity gene; JH gene, IgH junction gene; N, total number of mutations observed; R, replacement mutations; S, silent mutations; CDR, complementarity determining region; FR, framework region; P CDR, probability that excess or scarcity of the R mutations in the VH gene CDRs resulted from chance only; P FR, probability that excess or scarcity of the R mutations in the VH gene FRs resulted from chance only; NI, no gene identified with certainty; SC, stop codon.