Early IL-10 Effects on IL-2 Production by SEB-Stimulated PBMCs
| Hours . | IL-10 . | SEB . | IL-25-150 . | Fold Decrease . | |
|---|---|---|---|---|---|
| pg/mL . | U/mL5-151 . | ||||
| 6 | − | − | <85-152 | <0.085-152 | — |
| 12 | − | − | <8 | <0.08 | — |
| 18 | − | − | <8 | <0.08 | — |
| 24 | − | − | <8 | <0.08 | — |
| 6 | − | + | <8 | <0.08 | — |
| 12 | − | + | 172 ± 30.8 | 1.72 ± 0.308 | — |
| 18 | − | + | 816 ± 17.0 | 8.16 ± 0.170 | — |
| 24 | − | + | 1,375 ± 18.6 | 13.75 ± 0.186 | — |
| 6 | + | + | <8 | <0.08 | 0 |
| 12 | + | + | 53 ± 6.0 | 0.53 ± 0.060 | 3.2 |
| 18 | + | + | 53 ± 4.7 | 0.53 ± 0.047 | 15.4 |
| 24 | + | + | 96 ± 12.9 | 0.96 ± 0.129 | 14.3 |
| Hours . | IL-10 . | SEB . | IL-25-150 . | Fold Decrease . | |
|---|---|---|---|---|---|
| pg/mL . | U/mL5-151 . | ||||
| 6 | − | − | <85-152 | <0.085-152 | — |
| 12 | − | − | <8 | <0.08 | — |
| 18 | − | − | <8 | <0.08 | — |
| 24 | − | − | <8 | <0.08 | — |
| 6 | − | + | <8 | <0.08 | — |
| 12 | − | + | 172 ± 30.8 | 1.72 ± 0.308 | — |
| 18 | − | + | 816 ± 17.0 | 8.16 ± 0.170 | — |
| 24 | − | + | 1,375 ± 18.6 | 13.75 ± 0.186 | — |
| 6 | + | + | <8 | <0.08 | 0 |
| 12 | + | + | 53 ± 6.0 | 0.53 ± 0.060 | 3.2 |
| 18 | + | + | 53 ± 4.7 | 0.53 ± 0.047 | 15.4 |
| 24 | + | + | 96 ± 12.9 | 0.96 ± 0.129 | 14.3 |
IL-2 was measured from supernatants from PBMC cultures treated with media only, SEB (100 pg/mL) only, or IL-10 (10 U/mL) for 2 days before SEB (100 pg/mL). Cell supernatants were then collected after the indicated incubation period. Results were determined to be statistically significant by the Student’s t-test (P < .05).
IL-2 was measured in picograms per milliliter. The specific activity of the IL-2 standard was 1 × 107 U/mg and was used to calculate units per milliliter.
These results were below the detection limit of the assay.