IL-10 Effects on IL-2 Production by SEB-Stimulated PBMCs
| Days . | IL-10 . | SEB . | IL-24-150 . | Fold Decrease . | |
|---|---|---|---|---|---|
| pg/mL . | U/mL4-151 . | ||||
| 1.5 | − | − | 12 ± 5.0 | 0.12 ± 0.050 | — |
| 2.5 | − | − | <8‡ | <0.08‡ | — |
| 3.5 | − | − | <8 | <0.08 | — |
| 1.5 | − | + | 1,872 ± 185.0 | 18.72 ± 1.850 | — |
| 2.5 | − | + | 2,118 ± 246.0 | 21.18 ± 2.460 | — |
| 3.5 | − | + | 2,277 ± 318.0 | 22.77 ± 3.180 | — |
| 1.5 | + | + | 129 ± 6.0 | 1.29 ± 0.060 | 14.5 |
| 2.5 | + | + | 240 ± 29.0 | 2.40 ± 0.290 | 8.8 |
| 3.5 | + | + | 49 ± 7.0 | 0.49 ± 0.070 | 46.2 |
| Days . | IL-10 . | SEB . | IL-24-150 . | Fold Decrease . | |
|---|---|---|---|---|---|
| pg/mL . | U/mL4-151 . | ||||
| 1.5 | − | − | 12 ± 5.0 | 0.12 ± 0.050 | — |
| 2.5 | − | − | <8‡ | <0.08‡ | — |
| 3.5 | − | − | <8 | <0.08 | — |
| 1.5 | − | + | 1,872 ± 185.0 | 18.72 ± 1.850 | — |
| 2.5 | − | + | 2,118 ± 246.0 | 21.18 ± 2.460 | — |
| 3.5 | − | + | 2,277 ± 318.0 | 22.77 ± 3.180 | — |
| 1.5 | + | + | 129 ± 6.0 | 1.29 ± 0.060 | 14.5 |
| 2.5 | + | + | 240 ± 29.0 | 2.40 ± 0.290 | 8.8 |
| 3.5 | + | + | 49 ± 7.0 | 0.49 ± 0.070 | 46.2 |
IL-2 was measured from supernatants from PBMC cultures treated with media only, SEB (100 pg/mL) only, or IL-10 (10 U/mL) for 2 days before SEB (100 pg/mL). Cell supernatants were then collected after the indicated incubation period. Results were shown to be statistically significant by the Student’s t-test (P < .02).
IL-2 was measured in picograms per milliliter. The specific activity of the IL-2 standard was 1 × 107 U/mg and was used to calculate units per milliliter.
These results were below the detection limit of the assay.