Flow Cytometry Analysis of the Effect of IL-10 Addition at Various Times Relative to SEB Incubation on SEB-Stimulated CD4+ T Cells
| Treatment . | Cell Cycle Distribution (%)3-150 . | ||
|---|---|---|---|
| G1 . | S . | G2/M . | |
| None | >98 | <1 | <1 |
| IL-10 added 2 days before SEB | 84.50 | 11.70 | 3.80 |
| IL-10 added 1 day before SEB | 88.30 | 8.90 | 2.80 |
| IL-10 added same time as SEB | 83.50 | 13.00 | 3.50 |
| IL-10 added 1 day after SEB | 72.10 | 21.90 | 6.00 |
| IL-10 added 2 days after SEB | 70.90 | 22.80 | 6.30 |
| IL-10 added 3 days after SEB | 70.80 | 22.90 | 6.30 |
| IL-10 added 4 days after SEB | 65.80 | 26.80 | 7.40 |
| SEB only | 66.15 | 27.10 | 6.75 |
| Treatment . | Cell Cycle Distribution (%)3-150 . | ||
|---|---|---|---|
| G1 . | S . | G2/M . | |
| None | >98 | <1 | <1 |
| IL-10 added 2 days before SEB | 84.50 | 11.70 | 3.80 |
| IL-10 added 1 day before SEB | 88.30 | 8.90 | 2.80 |
| IL-10 added same time as SEB | 83.50 | 13.00 | 3.50 |
| IL-10 added 1 day after SEB | 72.10 | 21.90 | 6.00 |
| IL-10 added 2 days after SEB | 70.90 | 22.80 | 6.30 |
| IL-10 added 3 days after SEB | 70.80 | 22.90 | 6.30 |
| IL-10 added 4 days after SEB | 65.80 | 26.80 | 7.40 |
| SEB only | 66.15 | 27.10 | 6.75 |
PBMCs were treated with IL-10 (10 U/mL) at the times indicated and incubated with SEB (100 pg/mL). At 5.5 days after the addition of SEB, cells were collected and analyzed by flow cytometry. Data were gated to analyze CD4+ T cells.