Flow Cytometry Analysis of the Effect of Increasing Concentrations of IL-10 on SEB-Stimulated CD4+ T Cells
| IL-10 (U/mL) . | SEB . | Cell Cycle Distribution (%)* . | ||
|---|---|---|---|---|
| G1 . | S . | G2/M . | ||
| 0 | − | >98 | <1 | <1 |
| 30 | + | 80.50 | 16.05 | 3.45 |
| 10 | + | 75.10 | 21.00 | 3.85 |
| 3 | + | 73.80 | 21.60 | 4.50 |
| 0 | + | 61.50 | 32.00 | 6.50 |
| IL-10 (U/mL) . | SEB . | Cell Cycle Distribution (%)* . | ||
|---|---|---|---|---|
| G1 . | S . | G2/M . | ||
| 0 | − | >98 | <1 | <1 |
| 30 | + | 80.50 | 16.05 | 3.45 |
| 10 | + | 75.10 | 21.00 | 3.85 |
| 3 | + | 73.80 | 21.60 | 4.50 |
| 0 | + | 61.50 | 32.00 | 6.50 |
PBMCs were pretreated for 2 days with IL-10 at the indicated concentrations and then incubated with SEB (100 pg/mL) for 5.5 days before flow cytometry analysis. Data were gated to analyze CD4+ T cells.