Flow Cytometry Analysis of the Effect of IL-10 on SEB-Stimulated CD4+ T Cells
| Treatment . | Cell Cycle Distribution (%)* . | ||
|---|---|---|---|
| G1 . | S . | G2/M . | |
| Day 4 | |||
| None | >98 | <1 | <1 |
| SEB only | 58.80 ± 0.7 | 37.90 ± 0.3 | 3.25 ± 0.4 |
| IL-10 + SEB | 78.95 ± 2.2 | 19.00 ± 1.6 | 2.05 ± 0.6 |
| Day 5 | |||
| None | >98 | <1 | <1 |
| SEB only | 44.10 ± 1.0 | 47.15 ± 0.9 | 8.70 ± 0.1 |
| IL-10 + SEB | 72.95 ± 2.2 | 22.45 ± 1.8 | 4.70 ± 0.4 |
| Treatment . | Cell Cycle Distribution (%)* . | ||
|---|---|---|---|
| G1 . | S . | G2/M . | |
| Day 4 | |||
| None | >98 | <1 | <1 |
| SEB only | 58.80 ± 0.7 | 37.90 ± 0.3 | 3.25 ± 0.4 |
| IL-10 + SEB | 78.95 ± 2.2 | 19.00 ± 1.6 | 2.05 ± 0.6 |
| Day 5 | |||
| None | >98 | <1 | <1 |
| SEB only | 44.10 ± 1.0 | 47.15 ± 0.9 | 8.70 ± 0.1 |
| IL-10 + SEB | 72.95 ± 2.2 | 22.45 ± 1.8 | 4.70 ± 0.4 |
PBMCs were pretreated for 2 days with IL-10 (10 U/mL). SEB (100 pg/mL) was then added to these cultures and the incubation was continued for the indicated number of days before flow cytometry analysis. Data were gated to analyze CD4+ T cells. Results are expressed as the mean of duplicate samples ± SD. All differences between SEB only and IL-10 + SEB treatments were determined to be statistically significant by the Student’s t-test (P < .01).