Growth Inhibition of Human Breast Cancer Cell Lines and Fibroblasts by As2O3 and Ascorbic Acid
| Cell Line . | GST . | GSH . | Viable Cell (% of control) . | ||
|---|---|---|---|---|---|
| As2O3 . | AA . | As2O3 + AA . | |||
| T47D | 4.9 | 95.1 | 62.2 | 87.7 | 26.1 |
| MDA-MB-468 | 2.5 | 59.2 | 1.8 | 95.5 | 2.1 |
| RM5.21 | — | — | 103.0 | 89.2 | 100.9 |
| HEF | — | — | 101.0 | 93.2 | 105.0 |
| Cell Line . | GST . | GSH . | Viable Cell (% of control) . | ||
|---|---|---|---|---|---|
| As2O3 . | AA . | As2O3 + AA . | |||
| T47D | 4.9 | 95.1 | 62.2 | 87.7 | 26.1 |
| MDA-MB-468 | 2.5 | 59.2 | 1.8 | 95.5 | 2.1 |
| RM5.21 | — | — | 103.0 | 89.2 | 100.9 |
| HEF | — | — | 101.0 | 93.2 | 105.0 |
Cells were treated for 6 days with 1 μmol/L As2O3 or 62.5 μmol/L AA alone or combination. Viable cells number was determined by the MTT method.51 GST (nmol/mg protein/min) was determined according to reported method.14 GSH (nmol/mg protein) was determined as described in the Methods. Values are the mean of 3 independent experiments.
Abbreviations: RM5.21, human breast fibroblast; HEF, human embryo fibroblast.