Catalase and H2O2 on the Synergistic Effects of Ascorbic Acid With As2O3 in HL-60 Cells
| . | 1 Day . | 3 Days . | ||
|---|---|---|---|---|
| Growth Inhibition (%) . | Apoptosis (%) . | Growth Inhibition (%) . | Apoptosis (%) . | |
| Control | 0 | 2.3 ± 0.4 | 0 | 4.0 ± 1.3 |
| As2O3 | 3.0 ± 0.7 | 3.0 ± 1.7 | 15.2 ± 2.1 | 9.0 ± 1.7 |
| AA | 0 | 2.7 ± 1.3 | 0 | 5.0 ± 1.3 |
| AA + As2O3 | 27.0 ± 3.3 | 10.3 ± 3.0 | 69.7 ± 10.3 | 29.5 ± 1.7 |
| Dehydroascorbic acid + As2O3 | 8.3 ± 3.9 | 3.7 ± 2.1 | 11.6 ± 4.6 | 8.3 ± 0.9 |
| Catalase | 1.7 ± 1.0 | 3.0 ± 1.7 | 2.7 ± 1.3 | 4.3 ± 0.7 |
| Catalase + AA + As2O3 | 5.7 ± 1.3 | 3.3 ± 1.3 | 11.3 ± 3.0 | 10.0 ± 2.3 |
| H2O2 | 1.7 ± 0.3 | 3.3 ± 1.0 | 9.4 ± 3.3 | 11.0 ± 1.7 |
| H2O2 + As2O3 | 39.0 ± 3.3 | 16.3 ± 1.7 | 79.3 ± 5.1 | 44.6 ± 6.0 |
| . | 1 Day . | 3 Days . | ||
|---|---|---|---|---|
| Growth Inhibition (%) . | Apoptosis (%) . | Growth Inhibition (%) . | Apoptosis (%) . | |
| Control | 0 | 2.3 ± 0.4 | 0 | 4.0 ± 1.3 |
| As2O3 | 3.0 ± 0.7 | 3.0 ± 1.7 | 15.2 ± 2.1 | 9.0 ± 1.7 |
| AA | 0 | 2.7 ± 1.3 | 0 | 5.0 ± 1.3 |
| AA + As2O3 | 27.0 ± 3.3 | 10.3 ± 3.0 | 69.7 ± 10.3 | 29.5 ± 1.7 |
| Dehydroascorbic acid + As2O3 | 8.3 ± 3.9 | 3.7 ± 2.1 | 11.6 ± 4.6 | 8.3 ± 0.9 |
| Catalase | 1.7 ± 1.0 | 3.0 ± 1.7 | 2.7 ± 1.3 | 4.3 ± 0.7 |
| Catalase + AA + As2O3 | 5.7 ± 1.3 | 3.3 ± 1.3 | 11.3 ± 3.0 | 10.0 ± 2.3 |
| H2O2 | 1.7 ± 0.3 | 3.3 ± 1.0 | 9.4 ± 3.3 | 11.0 ± 1.7 |
| H2O2 + As2O3 | 39.0 ± 3.3 | 16.3 ± 1.7 | 79.3 ± 5.1 | 44.6 ± 6.0 |
As2O3 (1 μmol/L), ascorbic acid (62.5 μmol/L), catalase (500 U/mL), and H2O2 (4 μmol/L) were used for the experiments.