GSH Levels in NB4, su-DHL-4, and HL-60 Cells Treated With Different Agents for 24 Hours
| Cell . | Treatment . | GSH (nmol/mg protein) (mean ± SE) . |
|---|---|---|
| HL-60 | Basal | 51.4 ± 5.6 |
| BSO (100 μmol/L) | 13.7 ± 2.3 | |
| As2O3 (1 μmol/L) | 52.0 ± 3.1 | |
| AA (62.5 μmol/L) | 36.6 ± 4.0 | |
| As2O3 (1 μmol/L) + AA (62.5 μmol/L) | 34.6 ± 1.8 | |
| NB4 | Basal | 16.7 ± 1.8 |
| BSO (100 μmol/L) | 10.1 ± 1.4 | |
| AA (62.5 μmol/L) | 17.2 ± 2.7 | |
| As2O3 (1 μmol/L) | 16.5 ± 2.9 | |
| As2O3 (1 μmol/L) + AA (62.5 μmol/L) | 15.6 ± 1.0 | |
| NAC (10 mmol/L) | 27.2 ± 3.7 | |
| su-DHL-4 | Basal | 28.7 ± 2.2 |
| BSO (100 μmol/L) | 7.9 ± 1.3 | |
| AA (62.5 μmol/L) | 6.9 ± 2.6 | |
| As2O3 (1 μmol/L) | 24.1 ± 3.1 | |
| AA (62.5 μmol/L) + As2O3 (1 μmol/L) | 6.1 ± 1.8 |
| Cell . | Treatment . | GSH (nmol/mg protein) (mean ± SE) . |
|---|---|---|
| HL-60 | Basal | 51.4 ± 5.6 |
| BSO (100 μmol/L) | 13.7 ± 2.3 | |
| As2O3 (1 μmol/L) | 52.0 ± 3.1 | |
| AA (62.5 μmol/L) | 36.6 ± 4.0 | |
| As2O3 (1 μmol/L) + AA (62.5 μmol/L) | 34.6 ± 1.8 | |
| NB4 | Basal | 16.7 ± 1.8 |
| BSO (100 μmol/L) | 10.1 ± 1.4 | |
| AA (62.5 μmol/L) | 17.2 ± 2.7 | |
| As2O3 (1 μmol/L) | 16.5 ± 2.9 | |
| As2O3 (1 μmol/L) + AA (62.5 μmol/L) | 15.6 ± 1.0 | |
| NAC (10 mmol/L) | 27.2 ± 3.7 | |
| su-DHL-4 | Basal | 28.7 ± 2.2 |
| BSO (100 μmol/L) | 7.9 ± 1.3 | |
| AA (62.5 μmol/L) | 6.9 ± 2.6 | |
| As2O3 (1 μmol/L) | 24.1 ± 3.1 | |
| AA (62.5 μmol/L) + As2O3 (1 μmol/L) | 6.1 ± 1.8 |
Values are the mean of 3 independent experiments.
Abbreviations: BSO, buthionine sulfoxide; AA, ascorbic acid; NAC,N-acetylcysteine.