Effect of MoAbs to Various Chemokine Receptors on Migratory Capacity of Monocyte-Derived DCs
| Chemoattractant . | Treatment With . | No. of Migrated Cells . |
|---|---|---|
| Mean ± SD . | ||
| None | None | 7 ± 2 |
| RANTES | None | 111 ± 10 |
| Cont. IgG | 114 ± 5 | |
| αCCR-1 MoAb | 42 ± 4 | |
| αCCR-3 MoAb | 45 ± 7 | |
| αCCR-5 MoAb | 81 ± 7 | |
| αCXCR-4 MoAb | 108 ± 5 | |
| αCCR-1 MoAb/αCCR-3 MoAb | 24 ± 4 | |
| αCCR-1 MoAb/αCCR-5 MoAb | 35 ± 4 | |
| αCCR-3 MoAb/αCCR-5 MoAb | 33 ± 3 | |
| αCCR-1 MoAb/αCCR-3 MoAb/αCCR5 MoAb | 19 ± 3 |
| Chemoattractant . | Treatment With . | No. of Migrated Cells . |
|---|---|---|
| Mean ± SD . | ||
| None | None | 7 ± 2 |
| RANTES | None | 111 ± 10 |
| Cont. IgG | 114 ± 5 | |
| αCCR-1 MoAb | 42 ± 4 | |
| αCCR-3 MoAb | 45 ± 7 | |
| αCCR-5 MoAb | 81 ± 7 | |
| αCXCR-4 MoAb | 108 ± 5 | |
| αCCR-1 MoAb/αCCR-3 MoAb | 24 ± 4 | |
| αCCR-1 MoAb/αCCR-5 MoAb | 35 ± 4 | |
| αCCR-3 MoAb/αCCR-5 MoAb | 33 ± 3 | |
| αCCR-1 MoAb/αCCR-3 MoAb/αCCR5 MoAb | 19 ± 3 |
Monocyte-derived DCs (106) were pretreated with stated MoAbs (10 μg/mL) for 30 minutes at 37°C and seeded on the filters precoated on the lower surface with 5 μg gelatin. The RANTES (1 μg/mL) used as chemoattractants were added to the lower chamber. After 2 hours of incubation, the migrated cells on the lower surface were visually counted.