Rh123 Efflux and Its Inhibition in NK Cells
| . | Mean Channel Fluorescence of Rh123 . | % Inhibition of Rh123 Efflux . | ||||
|---|---|---|---|---|---|---|
| T0 . | T3 . | T3/CsA . | T3/PSC833 . | CsA . | PSC833 . | |
| Normal NK cells (n = 3) | 123.2 ± 14.6 | 19.1 ± 3.1 | 104.3 ± 12.6 | 102.7 ± 14.6 | 81.8 ± 0.9 | 80.2 ± 3.6 |
| Indolent NK-GLPD (n = 6) | 151.3 ± 11.9 | 34.6 ± 32.0 | 143.1 ± 14.9 | 141.5 ± 13.3 | 93.4 ± 3.1 | 91.7 ± 2.6 |
| Aggressive NK cell tumors (n = 3) | 131.8 ± 9.4 | 39.6 ± 18.9 | 72.9 ± 13.7 | 68.4 ± 20.8 | 36.9 ± 11.7 | 32.7 ± 10.1 |
| Cell line (n = 1) | 203.0 | 40.0 | 192.7 | 178.1 | 93.7 | 84.7 |
| . | Mean Channel Fluorescence of Rh123 . | % Inhibition of Rh123 Efflux . | ||||
|---|---|---|---|---|---|---|
| T0 . | T3 . | T3/CsA . | T3/PSC833 . | CsA . | PSC833 . | |
| Normal NK cells (n = 3) | 123.2 ± 14.6 | 19.1 ± 3.1 | 104.3 ± 12.6 | 102.7 ± 14.6 | 81.8 ± 0.9 | 80.2 ± 3.6 |
| Indolent NK-GLPD (n = 6) | 151.3 ± 11.9 | 34.6 ± 32.0 | 143.1 ± 14.9 | 141.5 ± 13.3 | 93.4 ± 3.1 | 91.7 ± 2.6 |
| Aggressive NK cell tumors (n = 3) | 131.8 ± 9.4 | 39.6 ± 18.9 | 72.9 ± 13.7 | 68.4 ± 20.8 | 36.9 ± 11.7 | 32.7 ± 10.1 |
| Cell line (n = 1) | 203.0 | 40.0 | 192.7 | 178.1 | 93.7 | 84.7 |
The values of mean channel fluorescence are shown after staining with Rh123 (T0), and after 3 hour efflux without inhibitors (T3), with inhibitors of 1 μmol/L CsA (T3/CsA), and of 1 μmol/L PSC 833 (T3/PSC833). Percent inhibition of efflux was calculated by incorporating Rh123 fluorescence values into the following formula: 100 (T3/CsA or T3/PSC833 − T0)/(T3 − T0).