Cell-Cycle Distribution
| Treatment 72 Hours . | Pre-G1 (Apoptosis) . | S + G2 + M (cycling cells) . | ||
|---|---|---|---|---|
| HUT . | CEM . | HUT . | CEM . | |
| Control | 5.9 | 7.9 | 49.4 | 51.3 |
| As | 19.4 | 8.5 | 35.7 | 54.3 |
| IFN 10 | 8.3 | 6 | 52.3 | 57.6 |
| IFN 10 + As | 45.8 | 18.4 | 37.7 | 53.6 |
| IFN 100 | 10 | 21.2 | 50.3 | 61.8 |
| IFN 100 + As | 4.5 | 31.5 | 43.6 | 54.5 |
| Treatment 72 Hours . | Pre-G1 (Apoptosis) . | S + G2 + M (cycling cells) . | ||
|---|---|---|---|---|
| HUT . | CEM . | HUT . | CEM . | |
| Control | 5.9 | 7.9 | 49.4 | 51.3 |
| As | 19.4 | 8.5 | 35.7 | 54.3 |
| IFN 10 | 8.3 | 6 | 52.3 | 57.6 |
| IFN 10 + As | 45.8 | 18.4 | 37.7 | 53.6 |
| IFN 100 | 10 | 21.2 | 50.3 | 61.8 |
| IFN 100 + As | 4.5 | 31.5 | 43.6 | 54.5 |
Effects of IFN, As, and their combination on the cell cycle distribution of leukemic cell lines HUT-102 and CEM. Histogram-related nuclear DNA contents was measured on flow cytometry and analyzed by Modfit software. The pre-G1 percentages represent apoptotic cells. Cycling cells, the sum of (S + G2 + M) phases, are a percentage of nonapoptotic cells.