Murine T-Cell Migration in Response to Soluble RANTES
| RANTES (ng/mL) . | No. of Migrating Cells . | |
|---|---|---|
| Recombinant RANTES . | HSVrantes . | |
| 0.1 | 16,041 ± 1,214 | 22,666 ± 1,527* |
| 1.0 | 26,833 ± 2,173* | 29,108 ± 4,741† |
| 10 | 26,666 ± 1,154† | 20,250 ± 1,299* |
| No. of Migrating Cells | ||
| HSVlac | Control | |
| Undiluted ND | 15,000 ± 1,500 | 11,916 ± 938 |
| 1:3 diluted ND | 11,500 ± 866 | |
| 1:9 diluted ND | 10,000 ± 1,732 | |
| RANTES (ng/mL) . | No. of Migrating Cells . | |
|---|---|---|
| Recombinant RANTES . | HSVrantes . | |
| 0.1 | 16,041 ± 1,214 | 22,666 ± 1,527* |
| 1.0 | 26,833 ± 2,173* | 29,108 ± 4,741† |
| 10 | 26,666 ± 1,154† | 20,250 ± 1,299* |
| No. of Migrating Cells | ||
| HSVlac | Control | |
| Undiluted ND | 15,000 ± 1,500 | 11,916 ± 938 |
| 1:3 diluted ND | 11,500 ± 866 | |
| 1:9 diluted ND | 10,000 ± 1,732 | |
Murine T cells (105) in X-Vivo 10 medium were placed in the upper well of a transwell chamber. Samples were either recombinant RANTES, HSVrantes-infected EL4 culture supernatant, or HSVlac-infected EL4 supernatant. All samples were diluted in X-Vivo 10 medium to get 10, 1, or 0.1 ng/mL of soluble RANTES and were added to the lower wells. Control wells contained only the X-Vivo 10 medium. The transwell chambers were incubated at 37°C for 4 hours, and cells that migrated to the lower wells were counted using a hemocytometer. The results are represented as the mean ± SD of the number of cells in the lower chamber in triplicate wells. P values were compared with the HSVlac control using the Student’s t-test.
Abbreviation: ND, not detected.
P ≤ .003.
P < .001.