Growth of MK in Liquid Culture From ET and PMF CD34+ Cells
| . | 0 . | PEG-rhuMGDF . | sMpl-Fc . | PEG-rhuMGDF + sMpl-Fc . |
|---|---|---|---|---|
| A | ||||
| t1 | 15 | 26 | 3 | 22 |
| t6 | 12 | 120 | 1 | 90 |
| t7 | 48 | 203 | 61 | 167 |
| t10 | 9 | 60 | 4 | 30 |
| t11 | 39 | 75 | 23 | 50 |
| t12 | 38 ± 6 | 77 ± 7 | 3 | 47 |
| t16 | 14 | 1,000 | 0 | 400 |
| t17 | 11 ± 1 | 67 ± 7 | 1.5 ± 0.5 | 26 ± 6 |
| t18 | 16 ± 4 | 101 ± 4 | 0.3 ± 0.5 | 57 ± 3 |
| m1 | 13 ± 1 | 196 ± 23 | 1 ± 0.5 | 64 ± 12 |
| m2 | 333 ± 230 | 1,700 ± 333 | 0 | 633 ± 152 |
| m3 | 53 ± 15 | 181 ± 83 | 12 ± 8 | 33 ± 11 |
| B | ||||
| t15 | 128 ± 18 | 441 ± 6 | 71 ± 15 | 233 ± 16 |
| m2 | 42 ± 2 | 260 ± 15 | 4 ± 0.5 | 178 ± 13 |
| m3 | 993 ± 205 | 3,746 ± 272 | 279 ± 55 | 1,039 ± 207 |
| . | 0 . | PEG-rhuMGDF . | sMpl-Fc . | PEG-rhuMGDF + sMpl-Fc . |
|---|---|---|---|---|
| A | ||||
| t1 | 15 | 26 | 3 | 22 |
| t6 | 12 | 120 | 1 | 90 |
| t7 | 48 | 203 | 61 | 167 |
| t10 | 9 | 60 | 4 | 30 |
| t11 | 39 | 75 | 23 | 50 |
| t12 | 38 ± 6 | 77 ± 7 | 3 | 47 |
| t16 | 14 | 1,000 | 0 | 400 |
| t17 | 11 ± 1 | 67 ± 7 | 1.5 ± 0.5 | 26 ± 6 |
| t18 | 16 ± 4 | 101 ± 4 | 0.3 ± 0.5 | 57 ± 3 |
| m1 | 13 ± 1 | 196 ± 23 | 1 ± 0.5 | 64 ± 12 |
| m2 | 333 ± 230 | 1,700 ± 333 | 0 | 633 ± 152 |
| m3 | 53 ± 15 | 181 ± 83 | 12 ± 8 | 33 ± 11 |
| B | ||||
| t15 | 128 ± 18 | 441 ± 6 | 71 ± 15 | 233 ± 16 |
| m2 | 42 ± 2 | 260 ± 15 | 4 ± 0.5 | 178 ± 13 |
| m3 | 993 ± 205 | 3,746 ± 272 | 279 ± 55 | 1,039 ± 207 |
Mean number of MKs ± SEM, quantified after 10 days of liquid culture with an inverted microscope (A) or by flow cytometry (B) after labeling with an R-PE or FITC-conjugated anti–CD-41 monoclonal antibody. Culture conditions were no growth factor (0), 10 ng/mL PEG-rhuMGDF, sMpl-Fc at 5 μg/mL, or a combination of sMpl-Fc (5 μg/mL) and PEG-rhuMGDF (10 ng/mL) (PEG-rhuMGDF + sMpl-Fc). Results are expressed for 1 × 103 CD34+ cells from TE (t) or PMF (m) patients.