Flow Cytometric Analysis of Peripheral Blood and Percolled Leukocyte Subpopulations (Percentage ± SD of Total Leukocytes)
| Cell Population . | Platelet Donors (n = 6) . | Percoll Preparation (n = 6) . | ||
|---|---|---|---|---|
| BALB/c Mice* (%) . | SCID Mice† (%) . | AsGm1-Treated SCID Mice‡ (%) . | BALB/c MC1-153 (%) . | |
| T cells (CD3+) | 40 ± 9 | <0.6 | <0.5 | 64 ± 7 |
| CD4+ T cells | 30 ± 7 | <0.3 | <0.3 | 50 ± 4 |
| CD8+ T cells | 10 ± 2 | <0.2 | <0.2 | 13 ± 1 |
| B cells (B220+) | 15 ± 6 | <0.3 | <0.3 | 18 ± 3 |
| NK cells (CD3−CD16+) | 9 ± 0.5 | 26 ± 7 | 4 ± 2 | 10 ± 3 |
| Monocytes (F4/80+) | 5 ± 0.6 | 10 ± 3 | 5 ± 2 | 6 ± 2 |
| Granulocytes (CD89+) | 28 ± 3 | 58 ± 54 | 77 ± 10 | <0.6 |
| Cell Population . | Platelet Donors (n = 6) . | Percoll Preparation (n = 6) . | ||
|---|---|---|---|---|
| BALB/c Mice* (%) . | SCID Mice† (%) . | AsGm1-Treated SCID Mice‡ (%) . | BALB/c MC1-153 (%) . | |
| T cells (CD3+) | 40 ± 9 | <0.6 | <0.5 | 64 ± 7 |
| CD4+ T cells | 30 ± 7 | <0.3 | <0.3 | 50 ± 4 |
| CD8+ T cells | 10 ± 2 | <0.2 | <0.2 | 13 ± 1 |
| B cells (B220+) | 15 ± 6 | <0.3 | <0.3 | 18 ± 3 |
| NK cells (CD3−CD16+) | 9 ± 0.5 | 26 ± 7 | 4 ± 2 | 10 ± 3 |
| Monocytes (F4/80+) | 5 ± 0.6 | 10 ± 3 | 5 ± 2 | 6 ± 2 |
| Granulocytes (CD89+) | 28 ± 3 | 58 ± 54 | 77 ± 10 | <0.6 |
Total peripheral blood leukocyte counts for BALB/c mice were 8.1 ± 2.2 × 103/μL.
Total peripheral blood leukocyte counts for SCID mice were 4.8 ± 1.2 × 103/μL.
Total peripheral blood leukocyte counts for anti-AsGm1–treated SCID mice were 4.1 ± 2.1 × 103/μL. SCID mice were injected IP with 100 μg antibody 72 hours before analysis.
BALB/c peripheral blood mononuclear cells (PBMC) were separated using 1.077 g/mL Percoll.