Morphology of EML Cells Treated 0 to 6 Days in SCF/atRA in the Presence and Absence of IL-3
| Day . | IL-3 . | Morphology . | ||
|---|---|---|---|---|
| Blasts/ Promyelocytes . | Myelocytes Segs/Bands . | Monocyte/ Macrophage . | ||
| 0 | − | 94% | 0 | 6% |
| + | 94% | 0 | 6% | |
| 2 | − | 94% | 0 | 6% |
| + | 78% | 13% | 9% | |
| 4 | − | 94% | 0 | 6% |
| + | 50% | 35% | 15% | |
| 6 | − | ND | ND | ND |
| + | 34% | 44% | 22% | |
| Day . | IL-3 . | Morphology . | ||
|---|---|---|---|---|
| Blasts/ Promyelocytes . | Myelocytes Segs/Bands . | Monocyte/ Macrophage . | ||
| 0 | − | 94% | 0 | 6% |
| + | 94% | 0 | 6% | |
| 2 | − | 94% | 0 | 6% |
| + | 78% | 13% | 9% | |
| 4 | − | 94% | 0 | 6% |
| + | 50% | 35% | 15% | |
| 6 | − | ND | ND | ND |
| + | 34% | 44% | 22% | |
EML cells were induced to undergo myeloid differentiation in the presence of SCF/IL-3 and atRA as described in Materials and Methods. The morphology of the cells was determined by examination of cytocentrifuge preparations of cells obtained at the indicated days that were stained with Giemsa. Percentages were determined by counting 100 to 500 cells per slide.
Abbreviation: ND, not determined.