Increased Numbers of Human Progenitors Detected in Secondary Recipients of Cells From Primary Recipients Adminsitered Human FL, SF, and IL-6
| Exp. . | No. of 1° Recipients per Group . | Proportion of BM Cells from 1° Recipients Injected into 2° Recipients . | Time of Assessment of 1° Recipients (wks posttransplant) . | Human Cell Type Evaluated in 2° Recipients . | 1° Recipients Administered no GF . | 1° Recipients Administered 3 GF . | ||
|---|---|---|---|---|---|---|---|---|
| No. of Positive 2° Recipients . | No. of Progenitors per 2° Recipients* . | No. of Positive 2° Recipients . | No. of Progenitors per 2° Recipients* . | |||||
| 1† | 4 | 25% | 4 | CFC | 1/4 | 3400 | 3/4 | 3400, 6100, 5100 |
| LTC-IC | 1/4 | 50 | 3/4 | 100, 280, 180 | ||||
| 2† | 3 | 25% | 4 | CFC | 0/4 | 1/3 | 14000 | |
| LTC-IC | 0/4 | 1/3 | 800 | |||||
| 2‡ | 3 | 25% | 4 | CFC | 3/4 | 9900, 16900, 3100 | ||
| LTC-IC | 2/4 | 400, 290 | ||||||
| Exp. . | No. of 1° Recipients per Group . | Proportion of BM Cells from 1° Recipients Injected into 2° Recipients . | Time of Assessment of 1° Recipients (wks posttransplant) . | Human Cell Type Evaluated in 2° Recipients . | 1° Recipients Administered no GF . | 1° Recipients Administered 3 GF . | ||
|---|---|---|---|---|---|---|---|---|
| No. of Positive 2° Recipients . | No. of Progenitors per 2° Recipients* . | No. of Positive 2° Recipients . | No. of Progenitors per 2° Recipients* . | |||||
| 1† | 4 | 25% | 4 | CFC | 1/4 | 3400 | 3/4 | 3400, 6100, 5100 |
| LTC-IC | 1/4 | 50 | 3/4 | 100, 280, 180 | ||||
| 2† | 3 | 25% | 4 | CFC | 0/4 | 1/3 | 14000 | |
| LTC-IC | 0/4 | 1/3 | 800 | |||||
| 2‡ | 3 | 25% | 4 | CFC | 3/4 | 9900, 16900, 3100 | ||
| LTC-IC | 2/4 | 400, 290 | ||||||
Total CFC or LTC-IC per mouse BM (individual values for each positive 2° recipient).
Daily—1 μg FL, 1 μg SF, 0.25 μg IL-6 per injection, every day for 1 month before sacrifice.
3 × wk—10 μg FL, 10 μg SF, 2 μg IL-6 per injection, 3 × week for 2 weeks before sacrifice. Note that the controls for this arm of the experiment are the same as for 2.