Sphingosine Formation Increases on PMN Granules and Plasma Membranes During Phagocytosis of EIgG
| Fractions . | Unstimulated: Sphingosine (pmol/106 cell equivalents) . | Phagocytosis: Sphingosine (pmol/106 cell equivalents) . |
|---|---|---|
| Azurophilic granules | 5.8 ± 1.5 | 21.8 ± 0.4 |
| Specific granules | 7.1 ± 0.9 | 23.9 ± 7.1 |
| Plasma membrane | 4.9 ± 1.2 | 15.8 ± 0.4 |
| Fractions . | Unstimulated: Sphingosine (pmol/106 cell equivalents) . | Phagocytosis: Sphingosine (pmol/106 cell equivalents) . |
|---|---|---|
| Azurophilic granules | 5.8 ± 1.5 | 21.8 ± 0.4 |
| Specific granules | 7.1 ± 0.9 | 23.9 ± 7.1 |
| Plasma membrane | 4.9 ± 1.2 | 15.8 ± 0.4 |
Control and phagocytosing cells were fractionated as described in Materials and Methods. The fractions were assayed for sphingosine content. Values represent the mean ± SD for three experiments (P < .001).