Expression of CXCR1 and CXCR2 on Human Neutrophils, Lymphocyte Subsets, and Eosinophils From Healthy Nonatopic Donors Stimulated by the Indicated Cytokines
| . | CXCR1 . | CXCR2 . |
|---|---|---|
| Eosinophils | ||
| Medium | 5.3 ± 0.3 | 4.8 ± 0.3 |
| 50 ng/mL IL-4 | 6.1 ± 0.4 | 5.6 ± 0.6 |
| 50 ng/mL IL-5 | 4.9 ± 0.4 | 4.4 ± 0.3 |
| 100 ng/mL TNFα | 5.5 ± 0.8 | 4.9 ± 0.8 |
| 30 ng/mL INFγ | 5.4 ± 0.6 | 5.0 ± 1.3 |
| 100 ng/mL GM-CSF | 4.7 ± 0.3 | 4.5 ± 0.3 |
| Isotype IgG2b | 4.9 ± 1.0 | 0 |
| Isotype IgG1 | 0 | 4.7 ± 0.3 |
| Neutrophils | ||
| Medium | 66.8 ± 10.6 | 73.7 ± 18.4 |
| Isotype IgG2b | 5.2 ± 1.2 | 0 |
| Isotype IgG1 | 0 | 4.6 ± 0.9 |
| Lymphocytes | ||
| Medium | 54.2 ± 10.4 | 41.6 ± 19.2 |
| Isotype IgG2b | 3.7 ± 0.6 | 0 |
| Isotype IgG1 | 0 | 4.6 ± 0.7 |
| . | CXCR1 . | CXCR2 . |
|---|---|---|
| Eosinophils | ||
| Medium | 5.3 ± 0.3 | 4.8 ± 0.3 |
| 50 ng/mL IL-4 | 6.1 ± 0.4 | 5.6 ± 0.6 |
| 50 ng/mL IL-5 | 4.9 ± 0.4 | 4.4 ± 0.3 |
| 100 ng/mL TNFα | 5.5 ± 0.8 | 4.9 ± 0.8 |
| 30 ng/mL INFγ | 5.4 ± 0.6 | 5.0 ± 1.3 |
| 100 ng/mL GM-CSF | 4.7 ± 0.3 | 4.5 ± 0.3 |
| Isotype IgG2b | 4.9 ± 1.0 | 0 |
| Isotype IgG1 | 0 | 4.7 ± 0.3 |
| Neutrophils | ||
| Medium | 66.8 ± 10.6 | 73.7 ± 18.4 |
| Isotype IgG2b | 5.2 ± 1.2 | 0 |
| Isotype IgG1 | 0 | 4.6 ± 0.9 |
| Lymphocytes | ||
| Medium | 54.2 ± 10.4 | 41.6 ± 19.2 |
| Isotype IgG2b | 3.7 ± 0.6 | 0 |
| Isotype IgG1 | 0 | 4.6 ± 0.7 |
Results are expressed as the mean channel fluorescence (MCF) and represent data from eight experiments. In the histogram for neutrophils and lymphocyte subsets, MCF was calculated on the second peak. Isotype controls are shown: IgG2b for anti-CXCR1 MoAb and IgG1 for anti-CXCR2 MoAb.